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  Proteomic tracking and analysis of a bacterial mixed culture

Kluge, S., Hoffmann, M., Benndorf, D., Rapp, E., & Reichl, U. (2012). Proteomic tracking and analysis of a bacterial mixed culture. Proteomics, 12(12), 1893-1901. doi:10.1002/pmic.201100362.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-8A56-8 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-A3F1-F
Genre: Journal Article

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 Creators:
Kluge, Sabine1, Author              
Hoffmann, Marcus2, Author              
Benndorf, Dirk2, Author              
Rapp, Erdmann2, Author              
Reichl, Udo2, 3, Author              
Affiliations:
1Otto-von-Guericke-Universität Magdeburg, External Organizations, ou_1738156              
2Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society, ou_1738140              
3Otto-von-Guericke-Universität Magdeburg, ou_1738156              

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Free keywords: bacterial mixed culture, population dynamics, cystic fibrosis, Ceftazidime, phenazine synthesis
 Abstract: To improve the understanding of microbial communities, experiments have to be performed, investigating community interactions. Therefore, a bacterial mixed culture was analyzed during batch cultivations with and without addition of the antibiotic Ceftazidime. The community was composed of Burkholderia cepacia, Pseudomonas aeruginosa, and Staphylococcus aureus, pathogens causing infections in cystic fibrosis patients. Gel-based proteomics and mass-spectrometry were used to obtain qualitative and quantitative proteomic data. Proteomic tracking, an approach for relative quantification of species-specific population dynamics of mixed cultures, was developed. During cultivation P. aeruginosa became dominant within the mixed culture while S. aureus was inhibited in growth. Analysis of samples - taken along cultivation - revealed about 270 differentially expressed proteins. Some of those proteins related to bacterial interactions, response to antibiotic treatment, and metabolic shifts. For instance, the enzymes PhzS and PhzG2 indicated the production of the antibiotic pigment pyocyanine by P. aeruginosa which is related to oxidative stress and therefore, might inhibit growth of S. aureus. Overall, the strategy applied not only allows species-specific tracking of the community composition but also provides valuable insights into the behavior of mixed cultures. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim [accessed November 2nd 2012]

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Language(s): eng - English
 Dates: 2012
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 626287
DOI: 10.1002/pmic.201100362
Other: 35/12
 Degree: -

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Title: Proteomics
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Weinheim, Fed. Rep. of Germany : WILEY-VCH Verlag
Pages: - Volume / Issue: 12 (12) Sequence Number: - Start / End Page: 1893 - 1901 Identifier: ISSN: 1615-9853
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000294310