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  Live attenuated influenza viruses produced in a suspension process with avian AGE1.CR.pIX cells

Lohr, V., Genzel, Y., Jordan, I., Katinger, D., Mahr, S., Sandig, V., et al. (2012). Live attenuated influenza viruses produced in a suspension process with avian AGE1.CR.pIX cells. BMC Biotechnology, 12, 79. doi:10.1186/1472-6750-12-79.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-8AA1-B Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-8C74-4
Genre: Journal Article

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628147_bmc_biotechnoloy.pdf (Publisher version), 336KB
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This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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 Creators:
Lohr, Verena1, Author              
Genzel, Yvonne1, Author              
Jordan, Ingo2, Author
Katinger, Dietmar3, Author
Mahr, Stefan4, Author              
Sandig, Volker2, Author
Reichl, Udo1, 5, Author              
Affiliations:
1Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society, ou_1738140              
2ProBioGen AG, Berlin, Germany , ou_persistent22              
3Polymun Scientific GmbH, Klosterneuburg, Austria, ou_persistent22              
4University for Applied Sciences, Furtwangen, Germany, ou_persistent22              
5Otto-von-Guericke-Universität Magdeburg, ou_1738156              

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Free keywords: Live attenuated influenza virus, Influenza vaccine production, Suspension cell culture, Cell density effect, AGE1.CR.pIX
 Abstract: Background: Current influenza vaccines are trivalent or quadrivalent inactivated split or subunit vaccines administered intramuscularly, or live attenuated influenza vaccines (LAIV) adapted to replicate at temperatures below body temperature and administered intranasally. Both vaccines are considered safe and efficient, but due to differences in specific properties may complement each other to ensure reliable vaccine coverage. By now, licensed LAIV are produced in embryonated chicken eggs. In the near future influenza vaccines for human use will also be available from adherent MDCK or Vero cell cultures, but a scalable suspension process may facilitate production and supply with vaccines. Results: We evaluated the production of cold-adapted human influenza virus strains in the duck suspension cell line AGE1.CR.pIX using a chemically-defined medium. One cold-adapted A (H1N1) and one cold-adapted B virus strain was tested, as well as the reference strain A/PR/8/34 (H1N1). It is shown that a medium exchange is not required for infection and that maximum virus titers are obtained for 1x10-6 trypsin units per cell. 1 L bioreactor cultivations showed that 4x106 cells/mL can be infected without a cell density effect achieving titers of 1x108 virions/mL after 24 h. Conclusions: Overall, this study demonstrates that AGE1.CR.pIX cells support replication of LAIV strains in a chemically-defined medium using a simple process without medium exchanges. Moreover, the process is fast with peak titers obtained 24 h post infection and easily scalable to industrial volumes as neither microcarriers nor medium replacements are required. © 2012 Lohr et al.; licensee BioMed Central Ltd. [accessed 2013 November 18th]

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Language(s): eng - English
 Dates: 2012
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 628147
DOI: 10.1186/1472-6750-12-79
Other: 39/12
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Title: BMC Biotechnology
Source Genre: Journal
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Publ. Info: BioMed Central
Pages: - Volume / Issue: 12 Sequence Number: - Start / End Page: 79 Identifier: ISSN: 1472-6750
CoNE: /journals/resource/111000136906066