English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  High-density microcarrier cell cultures for influenza virus production

Bock, A., Schulze-Horsel, J., Schwarzer, J., Rapp, E., Genzel, Y., & Reichl, U. (2011). High-density microcarrier cell cultures for influenza virus production. Biotechnology Progress, 27(1), 241-250. doi:10.1002/btpr.539.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-8CD1-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-A379-1
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Bock, A.1, Author              
Schulze-Horsel, J.1, Author              
Schwarzer, J.1, Author              
Rapp, E.1, Author              
Genzel, Y.1, Author              
Reichl, U.1, 2, Author              
Affiliations:
1Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society, ou_1738140              
2Otto-von-Guericke-Universität Magdeburg, ou_1738156              

Content

show
hide
Free keywords: -
 Abstract: Influenza virus A/PR/8/34 virus propagation in adherent Madin-Darby canine kidney cells in high-density microcarrier cultures is described. To improve virus yields perfusion and repeated fed-batch modes were applied using cell-specific feed rates. Cell densities up to 1.1 x 107 cells/mL were achieved. Cell-specific virus yields in high-density cultures were at similar level compared to standard, low-density cultivations. In the average 2400 and 3300 virions per cell were obtained for two variants of the virus strain A/PR/8/34, PR8-NIBSC and PR8-RKI, respectively. Maximum virus titer (HA-activity = 1778 HAU/100μL) for virus variant PR8-NIBSC was obtained for a cultivation infected before maximum cell concentration was reached. Copyright © 1999-2010 John Wiley & Sons, Inc. All Rights Reserved. [accessed December 3rd 2010]

Details

show
hide
Language(s): eng - English
 Dates: 2011
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 519942
DOI: 10.1002/btpr.539
Other: 47/10
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Biotechnology Progress
  Other : Biotechnol. Prog.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: New York, NY : American Institute of Chemical Engineers
Pages: - Volume / Issue: 27 (1) Sequence Number: - Start / End Page: 241 - 250 Identifier: ISSN: 8756-7938
CoNE: https://pure.mpg.de/cone/journals/resource/954925627362