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  Recombinant protein purification using gradient assisted simulated moving bed hydrophobic interaction chromatography - Part II: Process design and experimental validation

Gueorguieva, L., Palani, S., Rinas, U., Jayaraman, G., & Seidel-Morgenstern, A. (2011). Recombinant protein purification using gradient assisted simulated moving bed hydrophobic interaction chromatography - Part II: Process design and experimental validation. Journal of Chromatography A, 1218(37), 6402-6411. doi:10.1016/j.chroma.2011.07.008.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-8D67-8 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-B3C6-3
Genre: Journal Article

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 Creators:
Gueorguieva, L.1, Author              
Palani, S.2, 3, Author              
Rinas, U.4, 5, Author
Jayaraman, G.3, Author
Seidel-Morgenstern, A.1, 2, Author              
Affiliations:
1Otto-von-Guericke-Universität Magdeburg, External Organizations, ou_1738156              
2Physical and Chemical Foundations of Process Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society, ou_1738150              
3Indian Institute of Technology-Madras, Dep. of Biotechnology, Chennai, India, persistent:22              
4Leibniz University of Hanover, Institute of Technical Chemistry–Life Science, Hannover, Germany, ou_persistent22              
5Helmholtz Centre for Infection Research, Inhoffenstraße 7, 38124 Braunschweig, Germany, ou_persistent22              

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Free keywords: streptokinase; hydrophobic interaction chromatography; two-step gradient open-loop simulated moving bed chromatography
 Abstract: In the first part of this work adsorption isotherm parameters were acquired to describe the migration of recombinant streptokinase in Butyl Sepharose columns at different salt concentrations. Based on these results, a simulated moving bed (SMB) chromatographic process was designed and realised, which exploits a two–step salt gradient and allows the continuous separation of streptokinase from contaminants present in a clarified E. coli cell lysate solution. This second part describes the design of the three-zone open-loop gradient SMB process applying both equilibrium theory and an equilibrium stage model and presents results of a series of experiments aiming to obtain pure streptokinase. Moreover, the potential of the SMB process and the design approach are evaluated. Copyright © 2011 Published by Elsevier B.V. [accessed September 1st 2011]

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Language(s): eng - English
 Dates: 2011
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 570418
DOI: 10.1016/j.chroma.2011.07.008
 Degree: -

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Title: Journal of Chromatography A
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 1218 (37) Sequence Number: - Start / End Page: 6402 - 6411 Identifier: ISSN: 0021-9673
CoNE: https://pure.mpg.de/cone/journals/resource/954925527837_1