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  Model-based high cell density cultivation of Rhodospirillum rubrum under respiratory dark conditions

Zeiger, L., & Grammel, H. (2010). Model-based high cell density cultivation of Rhodospirillum rubrum under respiratory dark conditions. Biotechnology and Bioengineering, 105(4), 729-739. doi:10.1002/bit.22589.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-902B-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-F95F-C
Genre: Journal Article

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 Creators:
Zeiger, L.1, Author              
Grammel, H.1, Author              
Affiliations:
1Systems Biology, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society, ou_1738155              

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 Abstract: The potential of facultative photosynthetic bacteria as producers of photosynthetic pigments, vitamins, coenzymes and other valuable products has been recognized for decades. However, mass cultivation under photosynthetic conditions is generally inefficient due to the inevitable limitation of light supply when cell densities become very high. The previous development of a new cultivation process for maximal expression of photosynthetic genes under semi-aerobic dark conditions in common bioreactors offers a new perspective for utilizing the facultative photosynthetic bacterium Rhodospirillum rubrum for large-scale applications. Based on this cultivation system, the present study aimed in determining the maximal achievable cell density of R. rubrum in a bioreactor, thereby providing a major milestone on the way to industrial bioprocesses. As a starting point, we focus on aerobic growth due to higher growth rates and more facile process control under this condition, with the option to extend the process by an anaerobic production phase. Process design and optimization were supported by an unstructured computational process model, based on mixed-substrate kinetics. Key parameters for growth and process control were determined in shake-flask experiments or estimated by simulation studies. For fed-batch cultivation, a computer-controlled exponential feed algorithm in combination with a pH-stat element was implemented. As a result, a maximal cell density of 59 g cell dry weight (CDW) L-1 was obtained, representing so far not attainable cell densities for photosynthetic bacteria. The applied exponential fed-batch methodology therefore enters a range which is commonly employed for industrial applications with microbial cells. The biochemical analysis of high cell density cultures revealed metabolic imbalances, such as the accumulation and excretion of tetrapyrrole intermediates of the bacteriochlorophyll biosynthetic pathway John Wiley & Sons, Inc. All Rights Reserved. [accessed March 9, 2010]

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Language(s): eng - English
 Dates: 2010
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: eDoc: 439480
DOI: 10.1002/bit.22589
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Title: Biotechnology and Bioengineering
Source Genre: Journal
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Pages: - Volume / Issue: 105 (4) Sequence Number: - Start / End Page: 729 - 739 Identifier: -