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  A new MDCK suspension line cultivated in a fully defined medium in stirred-tank and wave bioreactor

Lohr, V., Genzel, Y., Behrendt, I., Scharfenberg, K., & Reichl, U. (2010). A new MDCK suspension line cultivated in a fully defined medium in stirred-tank and wave bioreactor. Vaccine, 28(38), 6256-6264. doi:10.1016/j.vaccine.2010.07.004.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-90B1-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-A27E-0
Genre: Journal Article

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 Creators:
Lohr, V.1, Author              
Genzel, Y.1, Author              
Behrendt, I.1, Author              
Scharfenberg, K.2, Author
Reichl, U.1, 3, Author              
Affiliations:
1Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society, ou_1738140              
2University of Applied Sciences Emden/Leer, Emden, ou_persistent22              
3Otto-von-Guericke-Universität Magdeburg, ou_1738156              

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Free keywords: Influenza virus Vaccine production Suspension MDCK Chemically defined medium Wave bioreactor
 Abstract: An adherently growing MDCK cell line was adapted in a two-step process in a fully defined medium and in suspension. The resulting MDCK.SUS2 cells were subsequently evaluated for their potential as host cells for influenza vaccine production in two lab-scale bioreactors (wave and stirred-tank). Cell concentrations up to 2.3×106 cells/mL were obtained after 96 h, which is slightly higher than cell concentrations obtained with adherent MDCK cells cultivated on microcarriers (2 g/L). Infections with influenza A/PR/8/34 and B/Malaysia resulted in high virus titers (2.90 and 2.75 logHAunits/100uL, respectively). The monitoring of extracellular metabolites, including amino acids, revealed a change in some of the metabolite consumption or release profiles, which indicates changes in metabolism during the adaptation process. Overall, the MDCK.SUS2 cell line represents a new cell substrate for a robust influenza vaccine production in a fully defined process. © 2010 Elsevier Ltd. All rights reserved. [accessed September 21st, 2010]

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Language(s): eng - English
 Dates: 2010
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 494474
Other: 23/10
DOI: 10.1016/j.vaccine.2010.07.004
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Title: Vaccine
Source Genre: Journal
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Publ. Info: Guildford, Surrey, UK : Elsevier
Pages: - Volume / Issue: 28 (38) Sequence Number: - Start / End Page: 6256 - 6264 Identifier: ISSN: 0264-410X
CoNE: /journals/resource/954925498091