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  Transcriptomic changes arising during light-induced sporulation in Physarum polycephalum

Barrantes Bustinza, I. W., Glockner, G., Meyer, S., & Marwan, W. (2010). Transcriptomic changes arising during light-induced sporulation in Physarum polycephalum. BMC Genomics, 11: 115. doi:10.1186/1471-2164-11-115.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-9169-1 Version Permalink: http://hdl.handle.net/21.11116/0000-0001-2316-F
Genre: Journal Article

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© 2010 Barrantes et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Barrantes Bustinza, Israel W.1, 2, Author              
Glockner, G.3, 4, 5, Author
Meyer, Sonja6, Author              
Marwan, Wolfgang6, Author              
Affiliations:
1International Max Planck Research School (IMPRS), Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society, ou_1738143              
2Otto-von-Guericke-Universität Magdeburg, External Organizations, ou_1738156              
3Leibniz Instiute, Jena, Germany, ou_persistent22              
4Universität Köln, Germany, ou_persistent22              
5Leibniz Institute for Freshwater Ecology, Berlin, Germany, ou_persistent22              
6Molecular Network Analysis, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society, ou_1738147              

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 Abstract: Background Physarum polycephalum is a free-living amoebozoan protist displaying a complex life cycle, including alternation between single- and multinucleate stages through sporulation, a simple form of cell differentiation. Sporulation in Physarum can be experimentally induced by several external factors, and Physarum displays many biochemical features typical for metazoan cells, including metazoan-type signaling pathways, which makes this organism a model to study cell cycle, cell differentiation and cellular reprogramming. Results In order to identify the genes associated to the light-induced sporulation in Physarum, especially those related to signal transduction, we isolated RNA before and after photoinduction from sporulation- competent cells, and used these RNAs to synthesize cDNAs, which were then analyzed using the 454 sequencing technology. We obtained 16,669 cDNAs that were annotated at every computational level. 13,169 transcripts included hit count data, from which 2,772 displayed significant differential expression (upregulated: 1,623; downregulated: 1,149). Transcripts with valid annotations and significant differential expression were later integrated into putative networks using interaction information from orthologs. Conclusions Gene ontology analysis suggested that most significantly downregulated genes are linked to DNA repair, cell division, inhibition of cell migration, and calcium release, while highly upregulated genes were involved in cell death, cell polarization, maintenance of integrity, and differentiation. In addition, cell death- associated transcripts were overrepresented between the upregulated transcripts. These changes are associated to a network of actin-binding proteins encoded by genes that are differentially regulated before and after light induction.

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Language(s): eng - English
 Dates: 2010
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: eDoc: 468542
DOI: 10.1186/1471-2164-11-115
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Title: BMC Genomics
  Alternative Title : BMC Genomics
Source Genre: Journal
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Pages: - Volume / Issue: 11 Sequence Number: 115 Start / End Page: - Identifier: -