English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Towards High Throughput Characterization of Glycosylation-Patterns Using a Capillary-DNA-Sequencer

Rapp, E., Schwarzer, J., Hennig, R., Roedig, J., & Reichl, U. (2009). Towards High Throughput Characterization of Glycosylation-Patterns Using a Capillary-DNA-Sequencer. Talk presented at CE in Biotechnology & Pharmaceutical Industries: 11th Symposium on the Practical Applications for the Analysis of Proteins, Nucleotides and Small Molecules (CE Pharm 2009). Boston, MA, USA. 2009-10-11 - 2009-10-15.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-91CD-0 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0025-0C4E-9
Genre: Talk

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Rapp, Erdmann1, Author              
Schwarzer, Jana1, Author              
Hennig, René1, Author              
Roedig, Jana1, Author              
Reichl, Udo1, 2, Author              
Affiliations:
1Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society, ou_1738140              
2Otto-von-Guericke-Universität Magdeburg, ou_1738156              

Content

show
hide
Free keywords: -
 Abstract: Within this work, the utilization of a capillary DNA-sequencer for N-glycan analysis [1] is presented. With this highly sensitive nano-separator, based on capillary gel electrophoresis with laser induced fluorescence detection (CGE-LIF), the N-glycans can be analyzed in two stages: first - comparing “glycan-pool fingerprints” and second - structural elucidation via database matching. The developed procedure allows monitoring and elucidation of N-glycosylation patterns of relevant glycoproteins. Besides workflow and proof of principle, suitability and potential of the method are depicted. The methods performance is exemplarily shown via elucidation of glycosylation patterns of pharmaceutical relevant glycoproteins (e.g. interferons, immunoglobulins, …). In more detail, feasibility and performance of this method is demonstrated exemplarily for hemagglutinin (HA), an important glycoprotein of the influenza virus membrane. The HA glycosylation patterns of different influenza virus strains, replicated in different mammalian cell lines for vaccine production were elucidated. Results concerning the influence of the host cell line on complexity and composition of the HA N-glycosylation pattern, are presented. Besides a strong host cell dependence of HA N-glycosylation that could be shown, a significant change in N-glycan type attached to HA was observed, comparing different virus types and subtypes [2]. [1] Schwarzer, J.; Rapp*, E.; Reichl, U. Electrophoresis, 2008, 29, 4203-4214. [2] Schwarzer, J.; Rapp*, E.; Hennig, R.; Genzel, Y.; Jordan, I.; Sandig, V.; Reichl U. Vaccine, 2009, in press.

Details

show
hide
Language(s): eng - English
 Dates: 2009
 Publication Status: Not specified
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: eDoc: 571791
 Degree: -

Event

show
hide
Title: CE in Biotechnology & Pharmaceutical Industries: 11th Symposium on the Practical Applications for the Analysis of Proteins, Nucleotides and Small Molecules (CE Pharm 2009)
Place of Event: Boston, MA, USA
Start-/End Date: 2009-10-11 - 2009-10-15

Legal Case

show

Project information

show

Source

show