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  Determination of the Adsorption Isotherm Parameters from Total Protein Mixture

Palani, S., Jayaraman, G., Gueorguieva, L., Rinas, U., & Seidel-Morgenstern, A. (2009). Determination of the Adsorption Isotherm Parameters from Total Protein Mixture. Talk presented at PREP 2009 - 22nd International Symposium, Exhibit & Workshops on Preparative / Process Chromatography, Ion Exchange, Adsorption / Desorption Processes & Related Separation Techniques. Philadelphia, USA. 2009-07-19 - 2009-07-22.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-9242-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-9243-D
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 Creators:
Palani, S.1, 2, Author              
Jayaraman, G.3, Author
Gueorguieva, L.1, 4, Author              
Rinas, U.3, Author
Seidel-Morgenstern, A.1, 4, Author              
Affiliations:
1Physical and Chemical Foundations of Process Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society, ou_1738150              
2Indian Institute of Technology-Madras, Dep. of Biotechnology, Chennai, India, persistent:22              
3Department of Biotechnology, Indian Institute of Technology Madras, Chennai 600 036, India Institut für Verfahrenstechnik, Otto-von-Guericke-Universität Magdeburg, Universitätsplatz 2, 39106 Magdeburg, Germany Helmholtz Zentrum für Infektionsforschung, Inhoffenstraße 7, 38124 Braunschweig, Germany, ou_persistent22              
4Otto-von-Guericke-Universität Magdeburg, External Organizations, ou_1738156              

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 Abstract: As a versatile tool, liquid chromatography has become an inevitable part of protein separation and purification processes. In the last two decades, chromatography has been very successfully applied for the purification of various target proteins from the mixtures of complex host cell proteins. In the last years, the continuous Simulated Moving Bed (SMB) process has started finding its applications for protein purification1. For the design of such SMB processes, the accurate estimation of adsorption isotherm parameters of the target components is very important. In the literature there are several methods describing the experimental determination of equilibrium isotherms of pure compounds and of complex mixtures2. Commonly the pure target protein of interest is not commercially available at an affordable price. In such cases an efficient direct estimation of the adsorption isotherms from the protein mixtures (fermentation broths or crude cell lysate) will decrease the process development time. Recombinant streptokinase is a FDA approved thrombolytic protein widely used for the treatment of congestive heart failure and peripheral vascular diseases. The purpose of this study is to explore the potential and applicability of the perturbation method3,4 for determining the adsorption isotherm parameters for recombinant streptokinase from crude cell lysate (multi-component mixture). The current state of the corresponding experimental work on recombinant streptokinase purification and the subsequent design of a related continuous SMB process capable to isolate the target protein will be presented. References: (1) Nicoud R M Subramanian G(ed.) in Bioseparation and Bioprocessing A hand book Vol I Wiley-VCH, Germany, 1998. (2) Seidel-Morgenstern A, J. Chromatogr. A 1037,(2004) 255. (3) Heuer C, Küsters E, Plattner T, Seidel-Morgenstern A, J. Chromatogr. A 827,(1998) 175. (4) Blumel C, Hugo P, Seidel-Morgenstern A, J. Chromatogr. A 865,(1999) 51.

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Language(s): eng - English
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 Identifiers: eDoc: 428525
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Title: PREP 2009 - 22nd International Symposium, Exhibit & Workshops on Preparative / Process Chromatography, Ion Exchange, Adsorption / Desorption Processes & Related Separation Techniques
Place of Event: Philadelphia, USA
Start-/End Date: 2009-07-19 - 2009-07-22

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