English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Purification of cell culture-derived influenza virus A/Puerto Rico/8/34 by membrane-based immobilized metal affinity chromatography

Opitz, L., Hohlweg, J., Reichl, U., & Wolff, M. W. (2009). Purification of cell culture-derived influenza virus A/Puerto Rico/8/34 by membrane-based immobilized metal affinity chromatography. Journal of Virological Methods, 161(2), 312-316. doi:10.1016/j.jviromet.2009.06.025.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-9397-7 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-A57A-D
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Opitz, L.1, Author              
Hohlweg, J.1, 2, Author              
Reichl, U.1, 3, Author              
Wolff, M. W.1, 3, Author              
Affiliations:
1Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society, ou_1738140              
2Fachhochschule Südwestfalen, Iserlohn, persistent:22              
3Otto-von-Guericke-Universität Magdeburg, ou_1738156              

Content

show
hide
Free keywords: -
 Abstract: The presented study focuses on the feasibility of immobilized metal affinity chromatography for purification of Madin Darby canine kidney cell culture-derived influenza virus particles. Therefore, influenza virus A/Puerto Rico/8/34 was screened for adsorption to different transition metal ions attached to iminodiacetic acid. Subsequently, capturing of the same virus strain using zinc-modified iminodiacetic acid membrane adsorbers was characterized regarding viral recoveries, host cell nucleic acid and total protein depletion as well as zinc ion leaching. In addition, the effect of the imidazole proton pump on virus stability was studied based on the hemagglutination activity. During adsorption in the presence of 1 M sodium chloride the majority of virus particles were recovered in the product (64 % Hemagglutination activity). Host cell nucleic acid and total protein content were reduced to approximately 7 % and 26%, respectively. This inexpensive and rapid method was applied reproducibly for influenza virus A/Puerto Rico/8/34 preparations on the laboratory scale. However, preliminary results with other virus strains indicated clearly a strong strain dependency for viral adsorption. Copyright © 2009 Elsevier B.V. All rights reserved. [Accessed September 16, 2009]

Details

show
hide
Language(s): eng - English
 Dates: 2009
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 436040
Other: 17/09 b
DOI: 10.1016/j.jviromet.2009.06.025
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Journal of Virological Methods
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 161 (2) Sequence Number: - Start / End Page: 312 - 316 Identifier: ISSN: 0166-0934
CoNE: /journals/resource/954925482627