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  Profiling of external metabolites during production of hantavirus nucleocapsid protein with recombinant Saccharomyces cerevisiae

Antoniukas, L., Grammel, H., Sasnauskas, K., & Reichl, U. (2008). Profiling of external metabolites during production of hantavirus nucleocapsid protein with recombinant Saccharomyces cerevisiae. Biotechnology Letters, 30(3), 415-420. doi:10.1007/s10529-007-9577-1.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-960E-9 Version Permalink: http://hdl.handle.net/21.11116/0000-0002-8551-C
Genre: Journal Article

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1756680_grammel.pdf (Publisher version), 221KB
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Antoniukas, L.1, 2, Author              
Grammel, H.3, Author              
Sasnauskas, K.1, Author
Reichl, U.2, 4, Author              
Affiliations:
1Institute of Biotechnology, Vilnius, Lithuania, ou_persistent22              
2Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society, ou_1738140              
3Systems Biology, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society, ou_1738155              
4Otto-von-Guericke-Universität Magdeburg, ou_1738156              

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 Abstract: Recombinant strains of Saccharomyces cerevisiae, producing hantavirus Puumala nucleocapsid protein for diagnostics and as a candidate vaccine were analyzed for uptake and excretion of intermediary metabolites during process optimization studies of fed-batch bioreactor cultures. Concentrations of glucose, maltose, galactose, pyruvate, acetaldehyde, ethanol, acetate, succinate and formaldehyde (used as a selection agent) were measured in the culture medium in order to find a metabolite pattern, indicative for the physiological state of the producer culture. When the inducer galactose was employed as a growth substrate, the metabolite profile of recombinant yeast cells was different from those of the non-recombinant original strain which excreted considerable amounts of metabolites with this substrate. In contrast, galactose-induced heterologous gene expression was indicated by the absence of excreted intermediary metabolites, except succinate. A model strain expressing a GFP fusion of hantavirus nucleocapsid protein differed in the excretion of metabolites from strains without GFP. In addition, the influence of alkali ions, employed for pH control is also demonstrated. © Springer Science+Business Media B.V. 2007 [accessed June 6th, 2008]

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Language(s): eng - English
 Dates: 2008
 Publication Status: Published in print
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 337096
DOI: 10.1007/s10529-007-9577-1
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Title: Biotechnology Letters
  Other : Biotechnol. Lett.
Source Genre: Journal
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Publ. Info: London [etc.] : Springer
Pages: - Volume / Issue: 30 (3) Sequence Number: - Start / End Page: 415 - 420 Identifier: ISSN: 0141-5492
CoNE: /journals/resource/954925471344