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  Analysis of Growth Dynamics in a Three-Species Chemostat by Disturbing Coexistence with Antibiotic Pulse

Riedele, C., Schmidt, J. K., & Reichl, U. (2007). Analysis of Growth Dynamics in a Three-Species Chemostat by Disturbing Coexistence with Antibiotic Pulse. Poster presented at European BioPerspectives 2007, Cologne, Germany.

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Riedele, C.1, Author           
Schmidt, J. K.1, Author           
Reichl, U.1, 2, Author           
1Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society, ou_1738140              
2Otto-von-Guericke-Universität Magdeburg, ou_1738156              


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 Abstract: From clinical data is known that treatment with antibiotics against Staphylococcus aureus, a bacterium that occurs in the first years in the life of CF-patients, leads to an earlier infection with the more pathogenic Pseudomonas aeruginosa [1]. The reasons for this effect are unknown. In our previous studies, a chemostat culture with three bacterial species relevant for cystic fibrosis (CF): P. aeruginosa, S. aureus and Burkholderia cepacia, was set up as a model system for microbial communities occurring in the lung of CF patients. In chemostat experiments with stationary conditions the coexistence of at least two of the species was observed. A quantitative T-RFLP method was adapted to monitor the specific cell number in mixed cultures of P. aeruginosa, S. aureus and B. cepacia [2]. The experimental work was supported by mathematical modelling, indicating a possible cause of coexistence on the basis of a food chain [3]. Based on this work and motivated by antibiotic therapies of CF patients, the dynamics of the mixed chemostat culture by setting antibiotic pulses will be investigated in the study presented here. The results from the model system will be compared to antibiotic pulse experiments in pure culture. This should lead to a better understanding of the behaviour of microbial communities on antibiotic treatment in contrast to pure cultures which are routinely used in antibiotic screening. It is expected that useful hints for the effect observed in antibiotic therapies of CF related infections can be derived from the results as well. First results of experiments are presented including establishment of quantification methods. Ceftazidim, the applied antibiotic, will be determined in culture broth by reversed phase HPLC. The susceptibility of ceftazidim to the different bacterial species will be tested, using a minimum inhibition concentration (MIC) assay. The adapted quantitative T-RFLP analyses will be further optimized in a way that intact but dead cells are separated from intact but viable cells and therefore do not appear in the calculation of the cell number. [1] Ratjen F., Döring G.: Lancet (2003), 361: 681-689 [2] Schmidt J.K., et al.: Biotechnology and Bioengineering, accepted Aug. 2006 [3] Heßeler J., et al.: Journal of Mathematical Biology (2006): 53: 556-584


Language(s): eng - English
 Dates: 2007
 Publication Status: Not specified
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 317137
 Degree: -


Title: European BioPerspectives 2007
Place of Event: Cologne, Germany
Start-/End Date: 2007-06-30 - 2007-07-01

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