English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Correlation between growth rates, EIIACrr phosphorylation, and intracellular cAMP levels in Escherichia coli K-12

Bettenbrock, K., Sauter, T., Jahreis, K., Kremling, A., Lengeler, J. W., & Gilles, E. D. (2007). Correlation between growth rates, EIIACrr phosphorylation, and intracellular cAMP levels in Escherichia coli K-12. Journal of Bacteriology, 189(19), 6891-6900. doi:10.1128/JB.00819-07.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-98DE-5 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-BE79-7
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Bettenbrock, K.1, Author              
Sauter, T.1, 2, Author              
Jahreis, K.3, Author
Kremling, A.1, Author              
Lengeler, J. W.3, Author
Gilles, E. D.1, Author              
Affiliations:
1Systems Biology, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society, ou_1738155              
2Univ. of Stuttgart, Inst. for System Dynamics and Control Engineering, Stuttgart, Germany, persistent:22              
3Univ. Osnabrück, AG Genetik, ou_persistent22              

Content

show
hide
Free keywords: -
 Abstract: In Escherichia coli K-12, components of the phosphoenolpyruvate-dependent phosphotransferase systems (PTSs) represent a signal transduction system involved in the global control of carbon catabolism through inducer exclusion, mediated by EIIACrr (=EIIAGlc), and catabolite repression, mediated by the cAMP∙CRP global regulator. We measured, in a systematic way, the relation between cellular growth rates and the key parameters of catabolite repression, i.e. the EIIACrr~P level and the cAMP levels, using in vitro and in vivo assays. Different growth rates were obtained by using either various carbon sources, or by growing the cells with limited concentrations of glucose, sucrose, and mannitol, in continuous bioreactor experiments. The ratio of EIIACrr to EIIACrr~P and the intracellular cAMP concentrations, deduced from the activity of a cAMP.CRP-dependent promoter, correlated well with specific growth rates between 0.3 h-1 and 0.7 h-1, corresponding to generation times of about 138 and 60 min, respectively. Below and above this range, these parameters were increasingly uncoupled from the growth rate, which perhaps indicates an increasing role executed by other global control systems, in particular the stringent-relaxed response system. Copyright © 2013 by the American Society for Microbiology. [accessed 2013 July 16th]

Details

show
hide
Language(s): eng - English
 Dates: 2007
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 320883
Other: 13/07
DOI: 10.1128/JB.00819-07
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Journal of Bacteriology
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 189 (19) Sequence Number: - Start / End Page: 6891 - 6900 Identifier: -