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  Step gradients in 3-zone simulated moving bed chromatography : Application to the purification of antibodies and bone morphogenetic protein-2

Keßler, L. C., Gueorguieva, L., Rinas, U., & Seidel-Morgenstern, A. (2007). Step gradients in 3-zone simulated moving bed chromatography: Application to the purification of antibodies and bone morphogenetic protein-2. Journal of Chromatography A, 1176(1-2), 69-78. doi:10.1016/j.chroma.2007.10.087.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-9930-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-B67D-D
Genre: Journal Article

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 Creators:
Keßler, L. C.1, Author              
Gueorguieva, L.1, 2, Author              
Rinas, U.3, Author
Seidel-Morgenstern, A.1, 2, Author              
Affiliations:
1Physical and Chemical Foundations of Process Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society, ou_1738150              
2Otto-von-Guericke-Universität Magdeburg, External Organizations, ou_1738156              
3Helmholtz Zentrum für Infektionsforschung GmbH, Abt. Strukturbiologie, Braunschweig, Germany, ou_persistent22              

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 Abstract: The simulated moving bed (SMB) technology is a proven tool for efficient separation of binary mixtures. However, relying on isocratic conditions limits the applicability of the classical SMB approach when considering the field of bioseparations. Here, the use of gradients opens up new possibilities. A gradient in a SMB process can be established by using different solvent strengths in the incoming feed and desorbent streams, resulting in two internal plateaus of elution strength. Thus, compared to the conventional process, the overall amount of solvent needed can be reduced, productivity can be increased and more concentrated product streams can be obtained. In this contribution, two case studies will be presented. At first, the separation of bovine IgG from lysozyme will be analyzed as a model system. Antibodies are a common target substance in bio-chromatography, as therapeutic monoclonal antibodies are among the most promising biopharmaceuticals. Using adsorption data obtained from single-column experiments, an appropriate SMB process was designed and implemented. The second target component is the active dimeric form of the bone morphogenetic protein-2 (BMP-2). This protein was isolated from a renaturation solution, which also contained its inactive monomeric form as well as other undefined proteins from the bacterial production strain. A 3-zone open-loop gradient-SMB approach was used successfully for both separations. Copyright © 2007 Elsevier Ltd. All rights reserved. [accessed 2013 November 26th]

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Language(s): eng - English
 Dates: 2007
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 335025
DOI: 10.1016/j.chroma.2007.10.087
Other: 31/07
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Title: Journal of Chromatography A
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 1176 (1-2) Sequence Number: - Start / End Page: 69 - 78 Identifier: ISSN: 0021-9673
CoNE: https://pure.mpg.de/cone/journals/resource/954925527837_1