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  Discontinuous and continuous separation of the monomeric and dimeric forms of human bone morphogenetic protein-2 from renaturation batches

Gueorguieva, L., Vallejo, L. F., Rinas, U., & Seidel-Morgenstern, A. (2006). Discontinuous and continuous separation of the monomeric and dimeric forms of human bone morphogenetic protein-2 from renaturation batches. Journal of Chromatography A, 1135(2), 142-150. doi:10.1016/j.chroma.2006.08.061.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-9963-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-B7CA-6
Genre: Journal Article

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 Creators:
Gueorguieva, L.1, 2, Author              
Vallejo, L. F.3, Author
Rinas, U.3, Author
Seidel-Morgenstern, A.1, 2, Author              
Affiliations:
1Physical and Chemical Foundations of Process Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society, ou_1738150              
2Otto-von-Guericke-Universität Magdeburg, External Organizations, ou_1738156              
3Gesellschaft für Biotechnologische Forschung, Braunschweig, Germany, ou_persistent22              

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Free keywords: Bone morphogenetic protein-2; Gradient elution; Continuous counter-current separation; Step gradients
 Abstract: Bone morphogenetic protein-2 (BMP-2) is one of the most interesting of the approximately 14 BMPs which belong to the transforming-growth-factor-β (TGF-β) superfamily. BMP-2 induces bone formation and thus plays an important role as a pharmaceutical protein. Recently, rhBMP-2 has been produced in form of inactive inclusion bodies in Escherichia coli. After solubilization and renaturation the biologically active dimeric form of rhBMP-2 can be generated. However, inactive monomers of BMP-2 are also formed during the renaturation process which must be separated from the active dimeric BMP-2. The purpose of this paper is to present: (a) results of an experimental study of a chromatographic separation of the monomeric and dimeric forms; and (b) a concept for a continuous counter-current simulated moving bed (SMB) process. The capacity of heparin as stationary phase was estimated for different salt concentrations in the mobile phase. A simulation study of a three-zone SMB process was performed applying a two step salt gradient. The results reveal the potential of the process for the purification of the dimeric BMP-2. © 2006 Published by Elsevier B.V. [accessed 2013 November 27th]

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Language(s): eng - English
 Dates: 2006
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 290788
DOI: 10.1016/j.chroma.2006.08.061
Other: 38/06
 Degree: -

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Title: Journal of Chromatography A
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 1135 (2) Sequence Number: - Start / End Page: 142 - 150 Identifier: ISSN: 0021-9673
CoNE: https://pure.mpg.de/cone/journals/resource/954925527837_1