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  Downstream Processes and Glycan Characterization of MDCK-Cell Derived Influenza Virus

Wolff, M. W., Kalbfuss, B., Lehmann, S., Morenweiser, R., Opitz, L., Zimmermann, A., et al. (2006). Downstream Processes and Glycan Characterization of MDCK-Cell Derived Influenza Virus. Talk presented at BioProcess Technology - Asia. Singapore. 2006-06-26 - 2006-06-28.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-9A21-E Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0025-0C09-5
Genre: Talk

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 Creators:
Wolff, M. W.1, 2, Author              
Kalbfuss, B.1, Author              
Lehmann, S.1, Author              
Morenweiser, R., Author
Opitz, L.1, Author              
Zimmermann, A.1, Author              
Reichl, U.1, 3, Author              
Affiliations:
1Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society, ou_1738140              
2Otto-von-Guericke-Universität Magdeburg, External Organizations, ou_1738156              
3Otto-von-Guericke-Universität Magdeburg, ou_1738156              

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 Abstract: Due to its annual death rate and the potential to cause pandemics, Influenza remains a major public health concern. Efforts to control the annual spread of influenza have centered on prophylactic vaccinations. Influenza viruses for human vaccine production are currently grown on embryonated eggs. However, this production process conveys major drawbacks such as lack of scalability, inability of some strains to replicate on eggs to high enough yields, and possible allergic reactions induced by egg proteins. These limitations emphasize the need for an alternative process. In recent years several continuous cell lines such as the Madin-Darby canine kidney (MDCK) have been successfully established for the production of influenza vaccines. These processes require the modification of existing downstream strategies to account for the modified upstream technology. The presented study focuses on the development of two downstream processing schemes for the purification of human Influenza A/PR/8/34 (H1N1) virus. Purification scheme 1 is based on a combination of size exclusion and anion exchange chromatography. Scheme 2 is centered on a lectin affinity chromatography. Both downstream processes represent a promising tool for the effective processing of MDCK-cell derived vaccines. In addition, the glycan analysis provides a powerful tool for the quality control of cell and egg derived antigens.

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Language(s): eng - English
 Dates: 2006
 Publication Status: Not specified
 Pages: -
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 Rev. Method: -
 Identifiers: eDoc: 327730
Other: Wolff2006
 Degree: -

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Title: BioProcess Technology - Asia
Place of Event: Singapore
Start-/End Date: 2006-06-26 - 2006-06-28

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