English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Limits and benefits of the interpretation of extracellular metabolite data in mammalian cell culture

Genzel, Y., & Reichl, U. (2006). Limits and benefits of the interpretation of extracellular metabolite data in mammalian cell culture. Talk presented at 1st Max Planck Workshop on "Metabolomics". Magdeburg, Germany. 2006-04-04 - 2006-04-05.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-9A4E-C Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0025-0C1F-6
Genre: Talk

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Genzel, Y.1, Author              
Reichl, U.1, 2, Author              
Affiliations:
1Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society, ou_1738140              
2Otto-von-Guericke-Universität Magdeburg, ou_1738156              

Content

show
hide
Free keywords: -
 Abstract: Before considering intracellular metabolites clear ideas on the extracellular conditions of experiments are obligatory. Many interesting questions on understanding metabolism of cells are generated under process conditions and not under defined steady state conditions with defined media. Here, we discuss limits and benefits of the interpretation of extracellular metabolite data for a process of influenza vaccine production in mammalian cells. Therefore, the production process is introduced together with relevant details on medium composition and cultivation conditions. Typical issues of metabolism during cell growth and viral infection are presented. Data on basic metabolites (glucose, lactate, glutamine, ammonia) are shown together with amino acid profiles measured from the cell culture medium [1]. Comparing different media (serum-containing, glutamine-free, serum-free [2-4]) as well as different cultivation methods (roller bottles, stirred tank bioreactor, wave bioreactor) it is demonstrated, what can be learned from extracellular metabolites and what questions remain. [1] Genzel Y.; König S.; Reichl U.; "Amino acid analysis in mammalian cell culture media containing serum and high glucose concentrations by anion exchange chromatography and integrated pulsed amperometric detection (IPAD)." , Anal. Biochem. 2004, 335, 119-125. [2] Genzel Y.; Behrendt I.; König S.; Sann H.; Reichl U.; "Metabolism of MDCK cells during cell growth and influence virus production in large-scale microcarrier culture" , Vaccine 2004, 22(17-18), 2202-2208. [3] Genzel Y.; Ritter JB.; König S.; Alt R.; Reichl U.; "Substitution of glutamine by pyruvate to reduce ammonia formation and growth inhibition of mammalian cells." , Biotechnol. Progr. 2005, 21 (1), 58-69. [4] Genzel Y.; Fischer M.; Reichl U.; "Serum-free influenza virus production avoiding washing steps and medium exchange in large-scale microcarrier culture.", Vaccine 2006, 24(16), 3261-3272.

Details

show
hide
Language(s): eng - English
 Dates: 2006
 Publication Status: Not specified
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: eDoc: 293556
 Degree: -

Event

show
hide
Title: 1st Max Planck Workshop on "Metabolomics"
Place of Event: Magdeburg, Germany
Start-/End Date: 2006-04-04 - 2006-04-05

Legal Case

show

Project information

show

Source

show