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  Continuous separation of the monomeric and dimeric forms of human bone morphogenetic protein-2 (BMP-2) using gradient Simulated Moving Bed (SMB) chromatography

Gueorguieva, L., Seidel-Morgenstern, A., Vallejo, L. F., & Rinas, U. (2004). Continuous separation of the monomeric and dimeric forms of human bone morphogenetic protein-2 (BMP-2) using gradient Simulated Moving Bed (SMB) chromatography. Poster presented at ISPPP 2004: International Symposium on the Separation of Proteins, Peptides and Polynucleotides, Aachen, Germany.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-9D81-9 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0025-6B22-2
Genre: Poster

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 Creators:
Gueorguieva, L.1, 2, Author              
Seidel-Morgenstern, A.1, 2, Author              
Vallejo, L. F.3, Author
Rinas, U.3, Author
Affiliations:
1Physical and Chemical Foundations of Process Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society, ou_1738150              
2Otto-von-Guericke-Universität Magdeburg, External Organizations, ou_1738156              
3Institute of Process Engineering, Otto von Guericke University, Universtaetsplatz 2, 39106 Magdeburg, Germany Gesellschaft für Biotechnologische Forschung, Mascheroder Weg 1, 38124 Braunschweig, Germany, ou_persistent22              

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 Abstract: Bone morphogenetic protein-2 (BMP-2) is one of the most interesting of the approx. 14 BMPs which belong to the transforming-growth-factor-β (TGF-β) superfamily. BMP-2 induces bone formation and thus plays an important role as a pharmaceutical protein. BMP-2 has been produced in form of inactive inclusion bodies in E. coli [1] and after solubilization and renaturation, the biologically active dimeric form of BMP-2 is generated [2]. However, inactive monomers of BMP-2 are also formed during the renaturation process which need to be separated from the active dimeric BMP-2. Goal of the project is the development of a continuous gradient SMB-process [3] exploiting two different salt concentrations in the feed solution and in the desorbent. A three-zone-configuration is designed based on a systematic study of the adsorption equilibrium of the monomers and dimers of BMP-2 at different salt concentrations. These equilibrium data allow to specify suitable salt concentrations and flow rates of the SMB-process offering the potential to collect the better adsorbed dimer with high purity at the extract port of the unit. In this presentation are presented: 1) A theoretical study of the three-zone-gradient-SMB process 2) The current state of the experimental work [1] Vallejo, L.F., Brokelmann, M., Marten, S., Trappe, S., Cabrera-Crespo, J., Hoffmann, A., Gross, G., Weich, H.A. and Rinas, U. (2002) Renaturation and purification of bone morphogenetic protein-2 produced as inclusion bodies in high-cell-density cultures of recombinant Escherichia coli. J. Biotechnol., 94, 185-194 [2] Vallejo, L.F. and Rinas, U. (2004) Optimized procedure for renaturation of recombinant human bone morphogenetic protein-2 at high protein concentration. Biotechnol. Bioeng., 85, 601-609 [3] Antos, D., Seidel-Morgenstern, A. (2001) Application of gradients in the simulated moving bed processes. Chem. Eng. Sci. 56, 6667-6682

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Language(s): eng - English
 Dates: 2004
 Publication Status: Not specified
 Pages: -
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 Rev. Method: -
 Identifiers: eDoc: 219303
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Title: ISPPP 2004 : International Symposium on the Separation of Proteins, Peptides and Polynucleotides
Place of Event: Aachen, Germany
Start-/End Date: 2004-10-19 - 2004-10-22

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