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  Amino acid analysis in mammalian cell culture based virus vaccine production processes

Genzel, Y., König, S., & Reichl, U. (2004). Amino acid analysis in mammalian cell culture based virus vaccine production processes. Talk presented at IICS: 17th annual International Ion Chromatography Symposium. Trier, Germany. 2004-09-20 - 2004-09-23.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-9DA6-8 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0025-0C23-7
Genre: Talk

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 Creators:
Genzel, Y.1, Author              
König, S.1, Author              
Reichl, U.1, 2, Author              
Affiliations:
1Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society, ou_1738140              
2Otto-von-Guericke-Universität Magdeburg, ou_1738156              

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 Abstract: Optimization strategies for bioprocesses often need a thorough analysis of substrate, product and metabolite concentrations. In mammalian cell culture especially the amino acid concentrations are of interest, as essential amino acids should not be limiting. When switching to defined protein and serum free media these profiles become even more important for process optimization. We are interested in virus vaccine production processes where the infection of mammalian cells with virus represents a stress situation with metabolic changes. To better understand these changes and to improve virus yields, we started analyzing amino acids from typical mammalian cell culture broth samples using direct separation by anion-exchange chromatography with integrated pulsed amperometric detection. Existing gradient elution conditions were adapted, considering additions of 1 vol% peptone and 10 vol% foetal calf serum (FCS) to the medium as well as changing concentrations of glucose from 5.5 g/L up to complete consumption as were typical for an influenza vaccine production process using Madin Darby Canine Kidney (MDCK) cells. Samples had to be analyzed in two dilutions with water (1: 33.3 and 1: 200) due to the strongly varying amino acid concentrations in the samples as a result of the medium composition and cell metabolism. Validation results and problems with oxidation or fouling of the gold electrode will be discussed. The amino acid profiles of the cell culture medium during three parallel cultivations of MDCK cells in roller bottles demonstrated the reproducibility of the method for a specific application.

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Language(s): eng - English
 Dates: 2004
 Publication Status: Not specified
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 207795
Other: 1011
 Degree: -

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Title: IICS : 17th annual International Ion Chromatography Symposium
Place of Event: Trier, Germany
Start-/End Date: 2004-09-20 - 2004-09-23

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