English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Glutamine-free media : use of pyruvate to avoid accumulation of ammonia in mammalian cell culture

Genzel, Y., Alt, R., & Reichl, U. (2004). Glutamine-free media: use of pyruvate to avoid accumulation of ammonia in mammalian cell culture. Poster presented at ESBES-5: European Symposium on Biochemical Engineering Science, Stuttgart, Germany.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-9DAA-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0025-6832-1
Genre: Poster

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Genzel, Y.1, Author              
Alt, R., Author
Reichl, U.1, 2, Author              
Affiliations:
1Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society, ou_1738140              
2Otto-von-Guericke-Universität Magdeburg, ou_1738156              

Content

show
hide
Free keywords: -
 Abstract: Glutamine is known to be one of the mayor energy sources in mammalian cell culture technology together with glucose. Different pathways for glutamine metabolism are possible, resulting in different energy and ammonia output. Ammonia can limit cell growth and product formation. Ideas to reduce ammonia formation are numerous. Here we present new aspects on the role of glutamine in mammalian cell culture media. We will show that the replacement of glutamine by pyruvate is supporting growth of different commercial cell lines (MDCK, BHK21, CHO) in serum containing and serum free media without ammonia production. No further additives, like peptides or hydrolysates are required. In addition, less lactate, another cell growth inhibitor is produced as less glucose is used. The use of this new medium formulation is presented in detail, considering the metabolism of MDCK cells in an influenza vaccine production process in roller bottles and large-scale microcarrier cultures as an example. The metabolite profiles for glucose, lactate, glutamine, ammonium, glutamate, pyruvate and extracellular amino acids will be analyzed. Metabolic pathways, which allow the cells to grow without glutamine, will be discussed. Further insights on the glutaminolysis are given by comparing metabolite profiles from variations of this new medium formulation. Additionally the impact on virus yield will be reported.

Details

show
hide
Language(s): eng - English
 Dates: 2004
 Publication Status: Not specified
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: eDoc: 207697
Other: 1016
 Degree: -

Event

show
hide
Title: ESBES-5 : European Symposium on Biochemical Engineering Science
Place of Event: Stuttgart, Germany
Start-/End Date: 2004-09-08 - 2004-09-11

Legal Case

show

Project information

show

Source

show