English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Monitoring and Control of high density cell culture systems

Bock, A., & Reichl, U. (2004). Monitoring and Control of high density cell culture systems. Poster presented at ESBES-5: European Symposium on Biochemical Engineering Science, Stuttgart, Germany.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-9DAC-B Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0025-6834-E
Genre: Poster

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Bock, A.1, Author              
Reichl, U.1, 2, Author              
Affiliations:
1Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society, ou_1738140              
2Otto-von-Guericke-Universität Magdeburg, ou_1738156              

Content

show
hide
Free keywords: -
 Abstract: Viral vaccines decrease the economic and social costs due to annual reoccurrence of worldwide flu infections. The aim of our research group is the development of integrated concepts to design and optimize cell growth and virus yield in vaccine production and the establishment of downstream processing methods to improve efficacy, purity and safety of vaccines. With a process of equine influenza virus production as an example we investigate: (a) Cultivation and scale-up of animal cells (MDCK) using microcarrier systems (b) Replication of influenza viruses (equine influenza Newmarket 1/93 H3N8)(c) Downstream processing to subunit or split vaccines. The process consists of a cell growth phase and a virus replication phase. Theoretical considerations have demonstrated that besides virus replication rate and survival of cells after infection, biomass concentration at time of infection has the main impact on virus yield. However, the increase in microcarrier concentration in the growth phase of the adhaerent MDCK cells to increase biomass concentration is only partly sucessful so far due to media limitations and inhibition effects. Therefore a detailed analysis of different metabolite concentrations e.g. lactate, ammonia, osmolality and the soluble concentrations of oxygen and carbon dioxide are essential for high density cell culture systems. Based on mathematical models for cell growth, virus replication, oxygen supply and carbon dioxide removal different control strategies will be established. A comparison of cultivation experiments shows the advantages of perfusion systems over the existing batch cultivations.

Details

show
hide
Language(s): eng - English
 Dates: 2004
 Publication Status: Not specified
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: eDoc: 207701
Other: 1019
 Degree: -

Event

show
hide
Title: ESBES-5 : European Symposium on Biochemical Engineering Science
Place of Event: Stuttgart, Germany
Start-/End Date: 2004-09-08 - 2004-09-11

Legal Case

show

Project information

show

Source

show