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  Cell physiology and metabolism : is glutamine really one of the main energy sources of mammalian cells?

Genzel, Y., Alt, R., & Reichl, U. (2004). Cell physiology and metabolism: is glutamine really one of the main energy sources of mammalian cells?. Poster presented at CCE IX (Cell Culture Engineering IX) 2004, Riviera Maya, Cancun, QR, Mexico.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-9E1D-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0025-6841-0
Genre: Poster

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 Creators:
Genzel, Y.1, Author              
Alt, R., Author
Reichl, U.1, 2, Author              
Affiliations:
1Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society, ou_1738140              
2Otto-von-Guericke-Universität Magdeburg, ou_1738156              

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 Abstract: In cell culture technology glutamine is known to be one of the mayor energy sources together with glucose. Different pathways for glutamine metabolism are possible, resulting in different energy and ammonium output. Ammonium can limit cell growth and product formation. Ideas to reduce ammonium formation are numerous. Here we present a new discussion on the glutaminolysis of mammalian cells. We will show that the replacement of glutamine by one single organic acid is supporting growth of different commercial cell lines (MDCK, BHK21, CHO) in serum containing and serum free media. No further additives, like peptides or hydrolysates are required. The use of this new medium formulation is presented in detail, considering the metabolism of MDCK cells in an influenza vaccine production process in roller bottles and microcarrier cultures as an example. The metabolite profiles for glucose, lactate, glutamine, ammonium, glutamate and extracellular amino acids will be analyzed. Additionally the impact on virus yield will be reported. How these cells can still grow and produce without glutamine will be discussed. Further insights on the glutaminolysis are given by comparing metabolite profiles from variations of this new medium formulation.

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Language(s): eng - English
 Dates: 2004
 Publication Status: Not specified
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: eDoc: 207807
Other: 1012
 Degree: -

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Title: CCE IX (Cell Culture Engineering IX) 2004
Place of Event: Riviera Maya , Cancun, QR, Mexico
Start-/End Date: 2004-03-07 - 2004-03-12

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