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  Glutamine-free media for vaccine production processes

Genzel, Y., Alt, R., & Reichl, U. (2005). Glutamine-free media for vaccine production processes. In F. Godia, & M. Fussenegger (Eds.), Animal Cell Technology meets Genomics: Proceedings ot the 18th ESACT Meeting (pp. 767-770).

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-A04A-0 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0025-B2D8-A
Genre: Conference Paper

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 Creators:
Genzel, Y.1, Author              
Alt, R., Author
Reichl, U.1, 2, Author              
Affiliations:
1Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society, ou_1738140              
2Otto-von-Guericke-Universität Magdeburg, ou_1738156              

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 Abstract: In cell culture technology high ammonia concentrations can limit cell growth and product formation, especially when focussing on higher cell densities. This high ammonia concentration normally results from glutamine consumption and decomposition. To overcome such problems we have tested several options and developed a glutamine-free medium for cell lines used in industrial applications. Preliminary investigations were carried out in static cultures of BHK 21, CHO and MDCK cells. Detailed characterization of MDCK cell metabolism was done for a vaccine production process of influenza virus (equine influenza Newmarket 1/93 H3N8) in MDCK cells using this newly developed glutamine-free medium: The results for cell growth and virus replication in a large-scale controlled microcarrier culture (5L bioreactor) are compared to the production in glutamine containing (2 mM) GMEM medium. Amino acid uptake and release will be compared with other on- & off-line data, to evaluate the importance of some amino acids in different phases of cell metabolism and virus replication with respect to lactate and ammonia production. © 2005 Springer. Printed in the Netherlands [accessed 2013 November 18th]

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Language(s): eng - English
 Dates: 2005
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: eDoc: 327741
DOI: 10.1007/1-4020-3103-3_159
 Degree: -

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Title: 18th ESACT Meeting
Place of Event: Granada, Spain
Start-/End Date: 2003-05-11 - 2003-05-14

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Title: Animal Cell Technology meets Genomics : Proceedings ot the 18th ESACT Meeting
Source Genre: Proceedings
 Creator(s):
Godia, F., Editor
Fussenegger, M., Editor
Affiliations:
-
Publ. Info: -
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 767 - 770 Identifier: -