English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Tools for the Glycome Analysis in the Post-Genomic Era

Rapp, E., Hennig, R., Borowiak, M., Kottler, R., & Reichl, U. (2011). Tools for the Glycome Analysis in the Post-Genomic Era. Talk presented at 7th ISABS Conference in Forensic, Anthropologic and Medical Genetics and Mayo Clinic Lectures in Translational Medicine. Bol, Island of Brač, Croatia. 2011-05-20 - 2011-05-24.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-A69B-E Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0025-0C81-4
Genre: Talk

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Rapp, E.1, Author              
Hennig, R.1, Author              
Borowiak, M.1, Author              
Kottler, R.1, Author              
Reichl, U.1, 2, Author              
Affiliations:
1Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society, ou_1738140              
2Otto-von-Guericke-Universität Magdeburg, ou_1738156              

Content

show
hide
Free keywords: -
 Abstract: Glycosylation is a post-translational modification that enriches protein complexity and function. Dysregulation of glycosylation is associated with a wide range of diseases, including cancer, diabetes, as well as congenital, cardiovascular, immunological and infectious disorders. With regard to biotechnology, proper glycosylation of biologicals is important, as deviations in glycosylation are known to be associated with adverse drug reactions and reduced therapeutic efficacy. Therefore, glycomics is a rapidly emerging field that can be viewed as a complement to other „omics“ approaches including proteomics and genomics. There is a dramatic dynamic increase in the demand for sophisticated databases and analytical tools in glycobiology respectively glycobiotechnology. However, glycomics is significantly lagging behind genomics and proteomics, mainly due to the absence of high-throughput analytical methods which can reliably quantify a multitude of glycan structures in complex biological samples. For that reason, the EU-wide collaborative project “Methods for high-throughput (HTP) analysis of protein glycosylation” is funded within the FP7 of the European Union and will be started in 2011. The project is designed to fill that technology gap through development of relevant, reliable, and affordable commercial HTP glycoanalysis tools. This modular glycoprofiling system – to be developed and established within the project – is based on three orthogonal analysis platforms (UPLC, MS and CGE-LIF). Project and analysis platforms will be presented, focussing on the glycoanalysis approach, based on multiplexed capillary gel electrophoresis with laser induced fluorescence detection (CGE-LIF) utilizing a DNA-sequencer, which shows high potential for high-throughput (HTP) glycoprofiling of glycoconjugates.

Details

show
hide
Language(s): eng - English
 Dates: 2011
 Publication Status: Not specified
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: eDoc: 571773
 Degree: -

Event

show
hide
Title: 7th ISABS Conference in Forensic, Anthropologic and Medical Genetics and Mayo Clinic Lectures in Translational Medicine
Place of Event: Bol, Island of Brač, Croatia
Start-/End Date: 2011-05-20 - 2011-05-24

Legal Case

show

Project information

show

Source

show