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  Complexin I plays a bilateral role in synaptic transmission during development at the calyx of Held synapse

Chang, S., Pedersen, M., Reim, K., & Taschenberger, H. (2013). Complexin I plays a bilateral role in synaptic transmission during development at the calyx of Held synapse. Biophysical Journal, 104(Supplement 1), 499A-500A. doi:10.1016/j.bpj.2012.11.2756.

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Chang, S.1, Author           
Pedersen, M.2, Author           
Reim, K., Author
Taschenberger, Holger1, Author                 
Affiliations:
1Research Group of Activity-Dependent and Developmental Plasticity at the Calyx of Held, MPI for biophysical chemistry, Max Planck Society, ou_578581              
2Emeritus Group of Membrane Biophysics, MPI for Biophysical Chemistry, Max Planck Society, ou_1571137              

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 Abstract: Complexins are small synaptic proteins which cooperate with the SNARE-complex during synaptic transmission. Different roles of complexins in the regulation of vesicle exocytosis have been proposed. Based on the results of genetic mutation or knock down/out studies, it is generally agreed that complexins are involved in vesicle priming and exocytosis during fast synchronous release and in clamping vesicles to prevent asynchronous release. However, depending on cell type, organism and experimental approach used, complexins appear to either facilitate or inhibit vesicle fusion. Here, we study the function of complexin I at the calyx of Held synapse. By taking advantage of the large size of the calyx terminal, allowing direct patch-clamp recordings, we investigate the consequences of the loss of function of complexin I. We demonstrate a developmentally aggravating phenotype of reduced EPSC amplitudes and enhanced asynchronous release. We provide evidence for a role of CPX I in recruiting Ca2+ channels to docked vesicles which may determine their release probability. The enhanced asynchronous release in complexin-deficient mice slowed-down the recovery of synchronous EPSCs after stimulus trains suggesting both, synchronous and asynchronous release events, were fed by a common pool of vesicles.

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Language(s): eng - English
 Dates: 2013-01-29
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.bpj.2012.11.2756
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Title: Biophysical Journal
  Other : Biophys. J.
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 104 (Supplement 1) Sequence Number: - Start / End Page: 499A - 500A Identifier: ISSN: 0006-3495
CoNE: https://pure.mpg.de/cone/journals/resource/954925385117