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  Transcriptional activation of the CrcZ and CrcY regulatory RNAs by the CbrB response regulator in Pseudomonas putida

García-Mauriño, S. M., Pérez-Martínez, I., Amador, C. I., Canosa, I., & Santero, E. (2013). Transcriptional activation of the CrcZ and CrcY regulatory RNAs by the CbrB response regulator in Pseudomonas putida. Molecular Microbiology, 89(1), 189-205. doi:10.1111/mmi.12270.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-AB4E-E Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-F6BC-E
Genre: Journal Article

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Garcia-Maurino_2013.pdf (Publisher version), 910KB
 
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García-Mauriño, Sofía Muñoz , Author
Pérez-Martínez, Isabel, Author
Amador, Cristina I.1, Author              
Canosa, Inés, Author
Santero, Eduardo, Author
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1Department Evolutionary Genetics, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445635              

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 Abstract: The CbrAB two-component system has been described as a high-ranked element in the regulatory hierarchy of Pseudomonas putida that controls a variety of metabolic and behavioural traits required for adaptation to changing environmental conditions. We show that the response regulatory protein CbrB, an activator of σN-dependent promoters, directly controls the expression of the small RNAs CrcZ and CrcY in P. putida. These two RNAs sequester the protein Crc, which is a translational repressor of multiple pathways linked to carbon catabolite repression. We characterized the in vivo and in vitro activation by CbrB at both crcZ and crcY promoters, and identified new DNA sequences where the protein binds. IHF, a co-activator at many σN-dependent promoters, also binds to the promoter regions and contributes to the activation of the sRNAs. CbrB phosphorylation is necessary at physiological activation conditions, but a higher dose of the protein allows in vitro transcriptional activation in its non-phosphorylated form. We also show there is some production of CrcY coming from an upstream promoter independent of CbrB. Thus, CbrAB constitute a global signal transduction pathway integrated in a higher regulatory network that also controls catabolite repression through the expression of the two regulatory RNAs CrcZ and CrcY.

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Language(s): eng - English
 Dates: 2013-05-172013-06-112013-07
 Publication Status: Published in print
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 Rev. Type: -
 Identifiers: DOI: 10.1111/mmi.12270
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Title: Molecular Microbiology
  Other : Mol. Microbiol.
Source Genre: Journal
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Publ. Info: Oxford : Blackwell Science
Pages: - Volume / Issue: 89 (1) Sequence Number: - Start / End Page: 189 - 205 Identifier: ISSN: 0950-382X (print)
ISSN: 1365-2958 (online)
CoNE: https://pure.mpg.de/cone/journals/resource/954925574950