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  Monitoring Homology Search during DNA Double-Strand Break Repair In Vivo

Renkawitz, J., Lademann, C. A., Kalocsay, M., & Jentsch, S. (2013). Monitoring Homology Search during DNA Double-Strand Break Repair In Vivo. MOLECULAR CELL, 50(2), 261-272. doi:10.1016/j.molcel.2013.02.020.

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 Creators:
Renkawitz, Jörg1, Author              
Lademann, Claudio A.1, Author              
Kalocsay, Marian1, Author              
Jentsch, Stefan1, Author              
Affiliations:
1Jentsch, Stefan / Molecular Cell Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565156              

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Free keywords: SACCHAROMYCES-CEREVISIAE; DONOR PREFERENCE; RECOMBINATION; RAD51; ORGANIZATION; ARCHITECTURE; PROTEINS; MOBILITY; GENOME; REGION
 Abstract: Homologous recombination (HR) is crucial for genetic exchange and accurate repair of DNA double-strand breaks and is pivotal for genome integrity. HR uses homologous sequences for repair, but how homology search, the exploration of the genome for homologous DNA sequences, is conducted in the nucleus remains poorly understood. Here, we use time-resolved chromatin immunoprecipitations of repair proteins to monitor homology search in vivo. We found that homology search proceeds by a probing mechanism, which commences around the break and samples preferentially on the broken chromosome. However, elements thought to instruct chromosome loops mediate homology search shortcuts, and centromeres, which cluster within the nucleus, may facilitate homology search on other chromosomes. Our study thus reveals crucial parameters for homology search in vivo and emphasizes the importance of linear distance, chromosome architecture, and proximity for recombination efficiency.

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Language(s): eng - English
 Dates: 2013-04-25
 Publication Status: Published in print
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: MOLECULAR CELL
Source Genre: Journal
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Publ. Info: 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA : CELL PRESS
Pages: - Volume / Issue: 50 (2) Sequence Number: - Start / End Page: 261 - 272 Identifier: ISSN: 1097-2765