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  Quantification issues of in vivo 1H NMR spectroscopy of the rat brain investigated at 16.4 T

Hong, S.-T., & Pohmann, R. (2013). Quantification issues of in vivo 1H NMR spectroscopy of the rat brain investigated at 16.4 T. NMR in Biomedicine, 26(1), 74-82. doi:10.1002/nbm.2821.

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Hong, S-T1, 2, Author              
Pohmann, R1, 2, Author              
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1Department High-Field Magnetic Resonance, Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497796              
2Max Planck Institute for Biological Cybernetics, Max Planck Society, Spemannstrasse 38, 72076 Tübingen, DE, ou_1497794              

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 Abstract: The accuracy and precision of the quantification of metabolite concentrations in in vivo1H NMR spectroscopy are affected by linewidth and signal-to-noise ratio (SNR). To study the effect of both factors in in vivo1H NMR spectra acquired at ultrahigh field, a reference spectrum was generated by summing nine in vivo1H NMR spectra obtained in rat brain with a STEAM sequence at 16.4 T. By progressive deterioration of linewidth and SNR, 6400 single spectra were generated. In an accuracy study, the variation in the mean concentrations of five metabolites was mainly dependent on SNR, whereas 11 metabolites were predominantly susceptible to the linewidth. However, the standard deviations of the concentrations obtained were dependent almost exclusively on the SNR. An insignificant correlation was found between most of the heavily overlapping metabolite peaks, indicating independent and reliable quantification. Two different approaches for the consideration of macromolecular signals were evaluated. The use of prior knowledge derived by parameterization of a metabolite-nulled spectrum demonstrated improved fitting quality, with reduced Cramér–Rao lower bounds, compared to the calculation of a regularized spline baseline.

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 Dates: 2013-01
 Publication Status: Published in print
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 Identifiers: DOI: 10.1002/nbm.2821
BibTex Citekey: HongP2012
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Title: NMR in Biomedicine
Source Genre: Journal
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Publ. Info: London : Heyden & Son
Pages: - Volume / Issue: 26 (1) Sequence Number: - Start / End Page: 74 - 82 Identifier: ISSN: 0952-3480
CoNE: https://pure.mpg.de/cone/journals/resource/954925574973