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  Tracing of noradrenergic projections using manganese-enhanced MRI

Eschenko, O., Evrard, H., Neves, R., Beyerlein, M., Murayama, Y., & Logothetis, N. (2012). Tracing of noradrenergic projections using manganese-enhanced MRI. NeuroImage, 59(4), 3252-3265. doi:10.1016/j.neuroimage.2011.11.031.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-B836-E Version Permalink: http://hdl.handle.net/21.11116/0000-0001-8A04-F
Genre: Journal Article

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Eschenko, O1, 2, Author              
Evrard, HC1, 2, Author              
Neves, RM1, 2, Author              
Beyerlein, M1, 2, Author              
Murayama, Y1, 2, Author              
Logothetis, NK1, 2, Author              
Affiliations:
1Department Physiology of Cognitive Processes, Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497798              
2Max Planck Institute for Biological Cybernetics, Max Planck Society, Spemannstrasse 38, 72076 Tübingen, DE, ou_1497794              

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 Abstract: We examined the applicability of manganese-enhanced MRI (MEMRI) to the in vivo tracing of diffuse neuromodulatory projections by means of simultaneous iontophoretic injections of an extremely low, non-toxic concentration of MnCl2 (10 mM) and fluorescent dextran in the locus coeruleus (LC) in the rat. We validated the use of the iontophoretic injection by reproducing previously reported results from pressure injections of MnCl2 in primary somatosensory cortex. Twenty four hours after injection in LC, Mn2 + labeling was detected in major cortical and subcortical targets of LC projections including predominantly ipsilateral primary motor and somatosensory cortices, hippocampus and amygdala. Although the injections were in most cases centered in the core of LC, the pattern of Mn2 + labeling greatly varied across rats. In addition, despite a certain degree of overlap of the labeling obtained with both MEMRI and classical tracing, MEMRI tracing consistently failed to reliably label not only several minor but also major targets of LC, notably the thalamus. The lack of Mn2 + labeling in thalamus possibly reflected a weaker functional connectivity within coeruleothalamic projections that could not be predicted by anatomical tracing. Inversely, a number of brain regions, particularly contralateral motor cortex, that were not or only sparsely labeled with fluorescent dextran were strongly labeled by Mn2 +. This discrepancy could be partly due to both the activity-dependent and transsynaptic nature of Mn2 + transport. The overall labeling produced using MEMRI with iontophoretic injections in LC indicates that the Mn2 + imaging of highly diffuse projections is in principle feasible. However, the labeling pattern of each individual case needs to be carefully interpreted particularly before submitting data for group analysis or in the case of longitudinal examination of discrete changes in functional connectivity under various physiological or behavioral conditions.

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 Dates: 2012-02
 Publication Status: Published in print
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 Identifiers: DOI: 10.1016/j.neuroimage.2011.11.031
BibTex Citekey: EschenkoENBML2011
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Title: NeuroImage
Source Genre: Journal
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Publ. Info: Orlando, FL : Academic Press
Pages: - Volume / Issue: 59 (4) Sequence Number: - Start / End Page: 3252 - 3265 Identifier: ISSN: 1053-8119
CoNE: /journals/resource/954922650166