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  Biocytin-derived MRI contrast agent for longitudinal brain connectivity studies

Mishra, A., Schüz, A., Engelmann, J., Beyerlein, M., Logothetis, N., & Canals, S. (2011). Biocytin-derived MRI contrast agent for longitudinal brain connectivity studies. ACS Chemical Neuroscience, 2(10), 578-587. doi:10.1021/cn200022m.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-B976-3 Version Permalink: http://hdl.handle.net/21.11116/0000-0001-AFE6-7
Genre: Journal Article

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https://pubs.acs.org/doi/10.1021/cn200022m (Publisher version)
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 Creators:
Mishra, A1, 2, Author              
Schüz, A1, 2, Author              
Engelmann, J2, 3, Author              
Beyerlein, M1, 2, Author              
Logothetis, NK1, 2, Author              
Canals, S1, 2, Author              
Affiliations:
1Department Physiology of Cognitive Processes, Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497798              
2Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497794              
3Department High-Field Magnetic Resonance, Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497796              

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 Abstract: To investigate the connectivity of brain networks noninvasively and dynamically, we have developed a new strategy to functionalize neuronal tracers and designed a biocompatible probe that can be visualized in vivo using magnetic resonance imaging (MRI). Furthermore, the multimodal design used allows combined ex vivo studies with microscopic spatial resolution by conventional histochemical techniques. We present data on the functionalization of biocytin, a well-known neuronal tract tracer, and demonstrate the validity of the approach by showing brain networks of cortical connectivity in live rats under MRI, together with the corresponding microscopic details, such as fibers and neuronal morphology under light microscopy. We further demonstrate that the developed molecule is the first MRI-visible probe to preferentially trace retrograde connections. Our study offers a new platform for the development of multimodal molecular imaging tools of broad interest in neuroscience, that capture in vivo the dynamics of large scale neural networks together with their microscopic characteristics, thereby spanning several organizational levels.

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 Dates: 2011-10
 Publication Status: Published in print
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 Rev. Method: -
 Identifiers: DOI: 10.1021/cn200022m
BibTex Citekey: MishraSEBLC2011
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Title: ACS Chemical Neuroscience
Source Genre: Journal
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Publ. Info: Washington, DC : American Chemical Society
Pages: - Volume / Issue: 2 (10) Sequence Number: - Start / End Page: 578 - 587 Identifier: ISSN: 1948-7193
CoNE: /journals/resource/1948-7193