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  Multimodal vessel mapping for precise large area alignment of functional optical imaging data to neuroanatomical preparations in marmosets

Valverde Salzmann, M., Wallace, D., Logothetis, N., & Schüz, A. (2011). Multimodal vessel mapping for precise large area alignment of functional optical imaging data to neuroanatomical preparations in marmosets. Journal of Neuroscience Methods, 201(1), 159-172. doi:10.1016/j.jneumeth.2011.07.029.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-BA28-9 Version Permalink: http://hdl.handle.net/21.11116/0000-0001-B1D2-9
Genre: Journal Article

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Valverde Salzmann, MF1, 2, 3, Author              
Wallace, DJ3, 4, Author              
Logothetis, NK1, 3, Author              
Schüz, A1, 3, Author              
Affiliations:
1Department Physiology of Cognitive Processes, Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497798              
2Department High-Field Magnetic Resonance, Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497796              
3Max Planck Institute for Biological Cybernetics, Max Planck Society, Spemannstrasse 38, 72076 Tübingen, DE, ou_1497794              
4Research Group Neural Population Imaging, Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497807              

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 Abstract: Imaging technologies, such as intrinsic optical imaging (IOI), functional magnetic resonance imaging (fMRI) or multiphoton microscopy provide excellent opportunities to study the relationship between functional signals recorded from a cortical area and the underlying anatomical structure. This, in turn, requires accurate alignment of the recorded functional imaging data with histological datasets from the imaged tissue obtained after the functional experiment. This alignment is complicated by distortions of the tissue which naturally occur during histological treatment, and is particularly difficult to achieve over large cortical areas, such as primate visual areas. We present here a method that uses IOI vessel maps revealed in the time course of the intrinsic signal, in combination with vascular casts and vascular lumen labeling techniques together with a pseudo three dimensional (p3D) reconstruction of the tissue architecture in order to facilitate alignment of IOI data with posthoc histological datasets. We demonstrate that by such a multimodal vessel mapping approach, we are able to constitute a hook in anatomical-functional data alignment that enables the accurate assignment of functional signals over large cortical regions. As an example, we present precise alignments of IOI responses showing orientation selectivity of primate V1 with anatomical sections stained for cytochrome-oxidase-reactivity.

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 Dates: 2011-09
 Publication Status: Published in print
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 Rev. Method: -
 Identifiers: DOI: 10.1016/j.jneumeth.2011.07.029
BibTex Citekey: ValverdeSalzmannWLS2011
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Title: Journal of Neuroscience Methods
  Other : J. Neurosci. Meth.
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 201 (1) Sequence Number: - Start / End Page: 159 - 172 Identifier: ISSN: 0165-0270
CoNE: /journals/resource/954925480594