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  Two-photon calcium imaging of evoked activity from L5 somatosensory neurons in vivo

Mittmann, W., Wallace, D., Czubayko, U., Herb, J., Schaefer, A., Looger, L., et al. (2011). Two-photon calcium imaging of evoked activity from L5 somatosensory neurons in vivo. Nature Neuroscience, 14(8), 1089-1093. doi:10.1038/nn.2879.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0013-BAC4-B Version Permalink: https://hdl.handle.net/21.11116/0000-0006-CA6B-F
Genre: Journal Article

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https://www.nature.com/articles/nn.2879.pdf (Publisher version)
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Mittmann, W1, 2, 3, Author           
Wallace, DJ1, 2, 3, Author           
Czubayko, U1, 2, 3, Author           
Herb, JT, Author
Schaefer, AT, Author
Looger, LL, Author
Denk , W, Author
Kerr, JND1, 2, 3, Author           
Affiliations:
1Former Research Group Network Imaging, Max Planck Institute for Biological Cybernetics, Max Planck Society, Spemannstrasse 38, 72076 Tübingen, DE, ou_2528697              
2Max Planck Institute for Biological Cybernetics, Max Planck Society, Spemannstrasse 38, 72076 Tübingen, DE, ou_1497794              
3Research Group Neural Population Imaging, Max Planck Institute for Biological Cybernetics, Max Planck Society, Spemannstrasse 38, 72076 Tübingen, DE, ou_1497807              

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 Abstract: Multiphoton imaging (MPI) is widely used for recording activity simultaneously from many neurons in superficial cortical layers in vivo. We combined regenerative amplification multiphoton microscopy (RAMM) with genetically encoded calcium indicators to extend MPI of neuronal population activity into layer 5 (L5) of adult mouse somatosensory cortex. We found that this approach could be used to record and quantify spontaneous and sensory-evoked activity in populations of L5 neuronal somata located as much as 800 μm below the pia. In addition, we found that RAMM could be used to simultaneously image activity from large (~80) populations of apical dendrites and follow these dendrites down to their somata of origin.

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 Dates: 2011-08
 Publication Status: Issued
 Pages: -
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 Rev. Type: -
 Identifiers: DOI: 10.1038/nn.2879
BibTex Citekey: MittmannWCHSLDK2011
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Title: Nature Neuroscience
  Other : Nat. Neurosci.
Source Genre: Journal
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Publ. Info: New York, NY : Nature America Inc.
Pages: - Volume / Issue: 14 (8) Sequence Number: - Start / End Page: 1089 - 1093 Identifier: ISSN: 1097-6256
CoNE: https://pure.mpg.de/cone/journals/resource/954925610931