English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Anterograde analysis of noradrenergic projections in the rat forebrain using classical and manganese-enhanced MRI (MEMRI) tract-tracing

Neves, R., Eschenko, O., Evrard, H., Beyerlein, M., & Logothetis, N. (2009). Anterograde analysis of noradrenergic projections in the rat forebrain using classical and manganese-enhanced MRI (MEMRI) tract-tracing. Poster presented at 10th Conference of Junior Neuroscientists of Tübingen (NeNa 2009), Ellwangen, Germany.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-C21E-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-C21F-4
Genre: Poster

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Neves, RM1, Author              
Eschenko, O1, Author              
Evrard, H1, 2, Author              
Beyerlein, M1, Author              
Logothetis, NK1, Author              
Affiliations:
1Department Physiology of Cognitive Processes, Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497798              
2Dept. Empirical Inference, Max Planck Institute for Intelligent System, Max Planck Society, ou_1497647              

Content

show
hide
Free keywords: -
 Abstract: We examined anterograde labeling of noradrenergic terminals originating from the neurons of brain stem neuromodulatory nucleus Locus Coeruleus (LC), a major course of noradrenaline in the rat forebrain, by means of simultaneous iontophoretic injection of paramagnetic (Mn2 +) and classical (fluorescent dextran) tracers in the LC. Both MEMRI and fluorescent microscopy revealed anterograde labeling in major terminal fields of LC neurons (Swanson and Hartman, 1975; Ungerstedt, 1971) 24 h and 5 d after injection, respectively. Predominantly ipsilateral labeling of thalamic nuclei, primary sensory cortices, medial prefrontal cortex, and olfactory bulbs reflected previously demonstrated monosynaptic projections of the LC neurons in multiple target brain regions. The labeling patterns of both paramagnetic and classical tracers were strikingly similar. Importantly, iontophoretic injection of Mn2+ did not produce neurotoxic effects as there were no signs of neuronal death or glial inflammatory reaction at the injection site 5 days after injection. The reported results further validate MEMRI tract-tracing technique allowing visualization a highly distributed and distal efferent projections arising from the brain stem nucleus. Thus, MEMRI may be used for mapping convergent target brain regions of different neuromodulatory systems in the same animal and their functional reorganization in longitudinal studies.

Details

show
hide
Language(s):
 Dates: 2009-11
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: URI: http://www.neuroschool-tuebingen-nena.de/
BibTex Citekey: NevesEEBL2009
 Degree: -

Event

show
hide
Title: 10th Conference of Junior Neuroscientists of Tübingen (NeNa 2009)
Place of Event: Ellwangen, Germany
Start-/End Date: -

Legal Case

show

Project information

show

Source

show