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  Automatic alignment of stacks of filament data

Dercksen, V., Weber, B., Gunther, D., Oberlaender, M., Prohaska, S., & Hege, H.-C. (2009). Automatic alignment of stacks of filament data. In 2009 IEEE International Symposium on Biomedical Imaging: From Nano to Macro (pp. 971-974). Piscataway, NJ, USA: IEEE.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0013-C3FD-8 Version Permalink: https://hdl.handle.net/21.11116/0000-0006-DAA1-E
Genre: Conference Paper

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https://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=5193216 (Publisher version)
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 Creators:
Dercksen, VJ, Author
Weber, B, Author
Gunther, D, Author
Oberlaender, M1, Author           
Prohaska, S, Author
Hege, H-C, Author
Affiliations:
1External Organizations, ou_persistent22              

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 Abstract: We present a fast and robust method for the alignment of image stacks containing filamentous structures. Such stacks are usually obtained by physical sectioning a specimen, followed by an optical sectioning of each slice. For reconstruction, the filaments have to be traced and the sub-volumes aligned. Our algorithm takes traced filaments as input and matches their endpoints to find the optimal transform. We show that our method is able to quickly and accurately align sub-volumes containing neuronal processes, acquired using brightfield microscopy. Our method also makes it possible to align traced microtubuli, obtained from electron tomography data, which are extremely difficult to align manually.

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 Dates: 2009-07
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1109/ISBI.2009.5193216
BibTex Citekey: DercksenWGOPH2009
 Degree: -

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Title: IEEE International Symposium on Biomedical Imaging: From Nano to Macro (ISBI '09)
Place of Event: Boston, MA, USA
Start-/End Date: 2009-06-28 - 2009-07-01

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Title: 2009 IEEE International Symposium on Biomedical Imaging: From Nano to Macro
Source Genre: Proceedings
 Creator(s):
Affiliations:
Publ. Info: Piscataway, NJ, USA : IEEE
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 971 - 974 Identifier: ISBN: 978-1-424-43931-7