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  CPP or cholesterol conjugation to antisense PNA for cellular delivery

Joshi, R., Mishra, R., Su, W., & Engelmann, J. (2008). CPP or cholesterol conjugation to antisense PNA for cellular delivery. In H. Lankinen, J. Vallivirta, T. Strandin, & J. Hepojoki (Eds.), Peptides 2008: Chemistry of Peptides in Life Science Technology and Medicine (pp. 550-551). Helsinki, Finland: FIPS.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-C737-F Version Permalink: http://hdl.handle.net/21.11116/0000-0003-3814-8
Genre: Conference Paper

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http://www.5z.com/30EPS/30EPS.pdf (Publisher version)
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 Creators:
Joshi, R1, 2, Author              
Mishra, R1, 2, Author              
Su, W1, 2, Author              
Engelmann, J1, 2, Author              
Affiliations:
1Former Department MRZ, Max Planck Institute for Biological Cybernetics, Max Planck Society, Spemannstrasse 38, 72076 Tübingen, DE, ou_2528700              
2Max Planck Institute for Biological Cybernetics, Max Planck Society, Spemannstrasse 38, 72076 Tübingen, DE, ou_1497794              

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 Abstract: Peptide nucleic acids (Proc Nat Acad Sci USA) are DNA mimics consisting of the four common bases as in DNA on a pseudopeptide backbone that makes them extremely stable in biological fluids. Antisense PNA can be targeted to mRNA in the cytoplasm in a complementary base pairing manner. In order to achieve efficient mRNA based targeting, endosomal release or direct uptake of Proc Nat Acad Sci USA into the cytosol is mandatory. However, relatively poor internalization of these agents is reported for most cells. 1 Several reports suggested cell penetrating peptide (CPP) based delivery systems for PNA delivery into cells. 2 Unfortunately, endosomal capture seems to be a major challenge in these approaches restricting the ability of PNA to bind with mRNA. Recent studies have explored the use of cholesterol-siRNA conjugates to enhance cellular import. 3 In order to attain efficient PNA internalization into cells, we synthesized two different sequences of cholesterol coupled antisense PNA and evaluated their uptake efficacy in comparison to a PNA-CPP conjugate previously reported by our group.

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 Dates: 2008-09
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: BibTex Citekey: 6760
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Title: Thirtieth European Peptide Symposium (Peptides 2008)
Place of Event: Helsinki, Finland
Start-/End Date: 2008-08-31 - 2008-09-05

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Title: Peptides 2008: Chemistry of Peptides in Life Science Technology and Medicine
Source Genre: Proceedings
 Creator(s):
Lankinen, H, Editor
Vallivirta, J, Editor
Strandin, T, Editor
Hepojoki, J, Editor
Affiliations:
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Publ. Info: Helsinki, Finland : FIPS
Pages: 719 Volume / Issue: - Sequence Number: 4-19-122 Start / End Page: 550 - 551 Identifier: ISBN: 978-952-92-7697-4