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  Single-spike detection in vitro and in vivo with a genetic Ca2+ sensor

Wallace, D., Meyer zum Alten Borgloh, S., Astori, S., Yang, Y., Bausen, M., Kügler, S., et al. (2008). Single-spike detection in vitro and in vivo with a genetic Ca2+ sensor. Nature methods, 5(9), 797-804. doi:10.1038/nmeth.1242.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-C797-A Version Permalink: http://hdl.handle.net/21.11116/0000-0003-2FDA-4
Genre: Journal Article

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Wallace, DJ1, 2, Author              
Meyer zum Alten Borgloh, S, Author
Astori, S, Author
Yang, Y, Author
Bausen, M, Author
Kügler, S, Author
Palmer, AE, Author
Tsien, RY, Author
Sprengel, R, Author
Kerr, JND2, 3, Author              
Denk, W, Author
Hasan, MT, Author
Affiliations:
1Research Group Neural Population Imaging, Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497807              
2Max Planck Institute for Biological Cybernetics, Max Planck Society, Spemannstrasse 38, 72076 Tübingen, DE, ou_1497794              
3Former Research Group Network Imaging, Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_2528697              

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 Abstract: Measurement of population activity with single-action-potential, single-neuron resolution is pivotal for understanding information representation and processing in the brain and how the brain‘s responses are altered by experience. Genetically encoded indicators of neuronal activity allow long-term, cell type–specific expression. Fluorescent Ca2+ indicator proteins (FCIPs), a main class of reporters of neural activity, initially suffered, in particular, from an inability to report single action potentials in vivo. Although suboptimal Ca2+-binding dynamics and Ca2+-induced fluorescence changes in FCIPs are important factors, low levels of expression also seem to play a role. Here we report that delivering D3cpv, an improved fluorescent resonance energy transfer–based FCIP, using a recombinant adeno-associated virus results in expression sufficient to detect the Ca2+ transients that accompany single action potentials. In upper-layer cortical neurons, we were able to detect transients associated with single action potentials firing at rates of

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 Dates: 2008-08
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.1038/nmeth.1242
BibTex Citekey: 5389
 Degree: -

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Title: Nature methods
  Other : Nature methods
Source Genre: Journal
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Publ. Info: New York, NY : Nature Pub. Group
Pages: - Volume / Issue: 5 (9) Sequence Number: - Start / End Page: 797 - 804 Identifier: ISSN: 1548-7091
CoNE: https://pure.mpg.de/cone/journals/resource/111088195279556