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  Single-spike detection in vitro and in vivo with a genetic Ca2+ sensor

Wallace, D., Meyer zum Alten Borgloh, S., Astori, S., Yang, Y., Bausen, M., Kügler, S., et al. (2008). Single-spike detection in vitro and in vivo with a genetic Ca2+ sensor. Nature methods, 5(9), 797-804. doi:10.1038/nmeth.1242.

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Wallace, DJ1, 2, 3, Autor           
Meyer zum Alten Borgloh, S, Autor
Astori, S, Autor
Yang, Y, Autor
Bausen, M, Autor
Kügler, S, Autor
Palmer, AE, Autor
Tsien, RY, Autor
Sprengel, R, Autor
Kerr, JND1, 2, 3, Autor           
Denk, W, Autor
Hasan, MT, Autor
Affiliations:
1Max Planck Institute for Biological Cybernetics, Max Planck Society, Spemannstrasse 38, 72076 Tübingen, DE, ou_1497794              
2Former Research Group Network Imaging, Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_2528697              
3Research Group Neural Population Imaging, Max Planck Institute for Biological Cybernetics, Max Planck Society, Spemannstrasse 38, 72076 Tübingen, DE, ou_1497807              

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 Zusammenfassung: Measurement of population activity with single-action-potential, single-neuron resolution is pivotal for understanding information representation and processing in the brain and how the brain‘s responses are altered by experience. Genetically encoded indicators of neuronal activity allow long-term, cell type–specific expression. Fluorescent Ca2+ indicator proteins (FCIPs), a main class of reporters of neural activity, initially suffered, in particular, from an inability to report single action potentials in vivo. Although suboptimal Ca2+-binding dynamics and Ca2+-induced fluorescence changes in FCIPs are important factors, low levels of expression also seem to play a role. Here we report that delivering D3cpv, an improved fluorescent resonance energy transfer–based FCIP, using a recombinant adeno-associated virus results in expression sufficient to detect the Ca2+ transients that accompany single action potentials. In upper-layer cortical neurons, we were able to detect transients associated with single action potentials firing at rates of

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 Datum: 2008-08
 Publikationsstatus: Erschienen
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 Ort, Verlag, Ausgabe: -
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 Identifikatoren: DOI: 10.1038/nmeth.1242
BibTex Citekey: 5389
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Titel: Nature methods
  Andere : Nature methods
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: New York, NY : Nature Pub. Group
Seiten: - Band / Heft: 5 (9) Artikelnummer: - Start- / Endseite: 797 - 804 Identifikator: ISSN: 1548-7091
CoNE: https://pure.mpg.de/cone/journals/resource/111088195279556