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  Towards extracellular Ca2+ sensing by MRI: synthesis and calcium-dependent 1H and 17O relaxation studies of two novel bismacrocyclic Gd3+ complexes.

Dhingra, K., Fousková, P., Angelovski, G., Maier, M., Logothetis, N., & Tóth, É. (2008). Towards extracellular Ca2+ sensing by MRI: synthesis and calcium-dependent 1H and 17O relaxation studies of two novel bismacrocyclic Gd3+ complexes. Journal of Biological Inorganic Chemistry, 13(1), 35-46. doi:10.1007/s00775-007-0296-9.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-CAAD-F Version Permalink: http://hdl.handle.net/21.11116/0000-0003-31A5-B
Genre: Journal Article

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Dhingra, K1, 2, Author              
Fousková, P, Author
Angelovski, G1, 2, Author              
Maier, ME, Author
Logothetis, NK1, 2, Author              
Tóth, É, Author
Affiliations:
1Department Physiology of Cognitive Processes, Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497798              
2Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497794              

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 Abstract: Two new bismacrocyclic Gd3+ chelates containing a specific Ca2+ binding site were synthesized as potential MRI contrast agents for the detection of Ca2+ concentration changes at the millimolar level in the extracellular space. In the ligands, the Ca2+-sensitive BAPTA-bisamide central part is separated from the DO3A macrocycles either by an ethylene (L1) or by a propylene (L2) unit [H4BAPTA is 1,2-bis(o-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid; H3DO3A is 1,4,7,10-tetraazacyclododecane-1,4,7-triacetic acid]. The sensitivity of the Gd3+ complexes towards Ca2+ and Mg2+ was studied by 1H relaxometric titrations. A maximum relaxivity increase of 15 and 10 was observed upon Ca2+ binding to Gd2L1 and Gd2L2, respectively, with a distinct selectivity of Gd2L1 towards Ca2+ compared with Mg2+. For Ca2+ binding, association constants of log K = 1.9 (Gd2L1) and log K = 2.7 (Gd2L2) were determined by relaxometry. Luminescence lifetime measurements and UV–vis spectrophotometry on the corresponding Eu3+ analogues proved that the complexes exist in the form of monohydrated and nonhydrated species; Ca2+ binding in the central part of the ligand induces the formation of the monohydrated state. The increasing hydration number accounts for the relaxivity increase observed on Ca2+ addition. A 1H nuclear magnetic relaxation dispersion and 17O NMR study on Gd2L1 in the absence and in the presence of Ca2+ was performed to assess the microscopic parameters influencing relaxivity. On Ca2+ binding, the water exchange is slightly accelerated, which is likely related to the increased steric demand of the central part leading to a destabilization of the Ln–water binding interaction.

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 Dates: 2008-01
 Publication Status: Published in print
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 Rev. Method: -
 Identifiers: DOI: 10.1007/s00775-007-0296-9
BibTex Citekey: 4695
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Title: Journal of Biological Inorganic Chemistry
Source Genre: Journal
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Publ. Info: Berlin : Springer
Pages: - Volume / Issue: 13 (1) Sequence Number: - Start / End Page: 35 - 46 Identifier: ISSN: 0949-8257
CoNE: https://pure.mpg.de/cone/journals/resource/954925573943