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  Phenotyping of Chondrocytes In Vivo and In Vitro Using cDNA Array Technology

Zien, A., Gebhard, P., Fundel, K., & Aigner, T. (2007). Phenotyping of Chondrocytes In Vivo and In Vitro Using cDNA Array Technology. Clinical Orthopaedics and Related Research, 460, 226-233. doi:10.1097/BLO.0b013e318047976a.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-CCA5-F Version Permalink: http://hdl.handle.net/21.11116/0000-0003-CB10-6
Genre: Journal Article

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https://insights.ovid.com/pubmed?pmid=17327807 (Publisher version)
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 Creators:
Zien, A1, 2, Author              
Gebhard, PM, Author
Fundel, K, Author
Aigner, T, Author
Affiliations:
1Department Empirical Inference, Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497795              
2Max Planck Institute for Biological Cybernetics, Max Planck Society, Spemannstrasse 38, 72076 Tübingen, DE, ou_1497794              

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 Abstract: The cDNA array technology is a powerful tool to analyze a high number of genes in parallel. We investigated whether large-scale gene expression analysis allows clustering and identification of cellular phenotypes of chondrocytes in different in vivo and in vitro conditions. In 100 of cases, clustering analysis distinguished between in vivo and in vitro samples, suggesting fundamental differences in chondrocytes in situ and in vitro regardless of the culture conditions or disease status. It also allowed us to differentiate between healthy and osteoarthritic cartilage. The clustering also revealed the relative importance of the investigated culturing conditions (stimulation agent, stimulation time, bead/monolayer). We augmented the cluster analysis with a statistical search for genes showing differential expression. The identified genes provided hints to the molecular basis of the differences between the sample classes. Our approach shows the power of modern bioinformatic algorithms for understanding and class ifying chondrocytic phenotypes in vivo and in vitro. Although it does not generate new experimental data per se, it provides valuable information regarding the biology of chondrocytes and may provide tools for diagnosing and staging the osteoarthritic disease process.

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 Dates: 2007-07
 Publication Status: Published in print
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 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1097/BLO.0b013e318047976a
BibTex Citekey: 3651
 Degree: -

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Title: Clinical Orthopaedics and Related Research
Source Genre: Journal
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Pages: - Volume / Issue: 460 Sequence Number: - Start / End Page: 226 - 233 Identifier: -