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  Synthesis and cellular uptake of a MR contrast agent coupled to an antisense peptide nucleic acid - cell- penetrating peptide conjugate

Su, W., Mishra, R., Pfeuffer, J., Wiesmüller, K., Ugurbil, K., & Engelmann, J. (2007). Synthesis and cellular uptake of a MR contrast agent coupled to an antisense peptide nucleic acid - cell- penetrating peptide conjugate. Contrast Media & Molecular Imaging, 2(1), 42-49. doi:10.1002/cmmi.126.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-CEB1-5 Version Permalink: http://hdl.handle.net/21.11116/0000-0003-D09B-3
Genre: Journal Article

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Su, W1, 2, Author              
Mishra, R1, 2, Author              
Pfeuffer, J1, 2, Author              
Wiesmüller , KH, Author
Ugurbil, K, Author
Engelmann, J1, 2, Author              
Affiliations:
1Former Department MRZ, Max Planck Institute for Biological Cybernetics, Max Planck Society, Spemannstrasse 38, 72076 Tübingen, DE, ou_2528700              
2Max Planck Institute for Biological Cybernetics, Max Planck Society, Spemannstrasse 38, 72076 Tübingen, DE, ou_1497794              

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 Abstract: In order to image mRNA transcription by in vivo magnetic resonance imaging (MRI), two intracellular MR contrast agents were developed, which are composed of a Gd-DOTA complex, a peptide nucleic acid (PNA) sequence and a cell-penetrating peptide. One was designed to bind to mRNA of dsRed (red fluorescent protein originating from Discosoma coral) by its PNA sequence, whereas the second one contains a nonsense sequence with no natural counterpart. The conjugates were synthesized using a continuous solid-phase synthesis scheme and characterized by ESI-MS. Fluorescence studies showed that both contrast agents could enter efficiently into 3T3 cells in a concentration-dependent manner from 0.5 to 9.0 µM. The contrast agent was located predominantly in vesicles around the nucleus, whereas no uptake into the nucleus was observed. The results of in vitro MR studies showed a statistically significant increase of the intracellular relaxation rate R 1,cell at a labeling concentration of only 0.5 µM, thus contrast enhanc ement was detectable too. These results suggest that the synthesized contrast agents could label cells for optical as well as MR imaging and in future might be useful to prove specific accumulation in cells containing target mRNA.

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 Dates: 2007-02
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1002/cmmi.126
BibTex Citekey: 4366
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Title: Contrast Media & Molecular Imaging
Source Genre: Journal
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Publ. Info: Hoboken, NJ : Wiley-Blackwell
Pages: - Volume / Issue: 2 (1) Sequence Number: - Start / End Page: 42 - 49 Identifier: ISSN: 1555-4309
CoNE: https://pure.mpg.de/cone/journals/resource/1555-4309