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  [Preparation of senkyunolide I by reversed-phase high performance liquid chromatography]

Zhang, X., Xu, Q., Xiao, H., & Liang, X. (2004). [Preparation of senkyunolide I by reversed-phase high performance liquid chromatography]. Se Pu: Chinese Journal of Chromatograph, 22(1), 41-43.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-DA37-9 Version Permalink: http://hdl.handle.net/21.11116/0000-0005-5000-0
Genre: Journal Article

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Zhang, X1, Author              
Xu , Q, Author
Xiao, H, Author
Liang, X, Author
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1External Organizations, ou_persistent22              

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 Abstract: A method for the isolation of senkyunolide I from Chuanxiong with reversed-phase preparative high performance liquid chromatography (HPLC) is described. After 95 ethanol extract was obtained with solvent extraction and normal-phase silica column separation, a fraction containing senkyunolide I was acquired and subjected to preparative HPLC for the isolation of target component. In the experiment a solvent system consisting of methanol-0.05 mol/L NH4Ac aqueous solution was found effective to eliminate the interference of ferulic acid that was the major compound in the sample. Senkyunolide I and ferulic acid were separated completely by using a stepwise gradient solvent system of 10 methanol in 10 min, and 60 methanol in 10 - 40 min. The subfractions were collected and subjected to an open MCI gel CHP-20P column for desalination respectively. Senkyunolide I was obtained and the structure was identified by mass spectrometry (MS) and nuclear magnetic resonance (NMR). This method is effective and reliable for t he preparation of senkyunolide I from Chuanxiong.

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 Dates: 2004-01
 Publication Status: Published in print
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 Identifiers: BibTex Citekey: 4448
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Title: Se Pu: Chinese Journal of Chromatograph
Source Genre: Journal
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Pages: - Volume / Issue: 22 (1) Sequence Number: - Start / End Page: 41 - 43 Identifier: -