English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Comparison of the Effects of Cyclosporin A on the Metabolism of Perfused Rat Brain Slices During Normoxia and Hypoxia

Serkova, N., Donohoe, P., Gottschalk, S., Hainz, C., Neumann, C., Bickler, P., et al. (2002). Comparison of the Effects of Cyclosporin A on the Metabolism of Perfused Rat Brain Slices During Normoxia and Hypoxia. Journal of Cerebral Blood Flow and Metabolism, 22(3), 342-352. doi:10.1097/00004647-200203000-00012.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Serkova, N, Author
Donohoe , P, Author
Gottschalk, S1, Author              
Hainz, C, Author
Neumann, CU, Author
Bickler, PE, Author
Benet, LZ, Author
Leibfritz, D, Author
Christians, U, Author
Affiliations:
1External Organizations, ou_persistent22              

Content

show
hide
Free keywords: -
 Abstract: The authors evaluated and compared the metabolic effects of cyclosporin A in the rat brain during normoxia and hypoxia/reperfusion. Ex vivo 31P magnetic resonance spectroscopy experiments based on perfused rat brain slices showed that under normoxic conditions, 500 mug/L cyclosporin A significantly reduced mitochondrial energy metabolism (nucleotide triphosphate, 83 plusminus 9 of controls; phosphocreatine, 69 plusminus 9) by inhibition of the Krebs cycle (glutamate, 77 plusminus 5) and oxidative phosphorylation (NAD+, 65 plusminus 14) associated with an increased generation of reactive oxygen species (285 plusminus 78 of control). However, the same cyclosporin A concentration (500 mug/L) was found to be the most efficient concentration to inhibit the hypoxia-induced mitochondrial release of Ca2+ in primary rat hippocampal cells with cytosolic Ca2+ concentrations not significantly different from normoxic controls. Addition of 500 mug/L cyclosporin A to the perfusion medium protected high-energy phosphate metabolism (nucleotide triphosphate, 11 plusminus 15 of control vs. 35 plusminus 9 with 500 mug/L cyclosporin A) and the intracellular pH (6.2 plusminus 0.1 control vs. 6.6 plusminus 0.1 with cyclosporin A) in rat brain slices during 30 minutes of hypoxia. Results indicate that cyclosporin A simultaneously decreases and protects cell glucose and energy metabolism. Whether the overall effect was a reduction or protection of cell energy metabolism depended on the concentrations of both oxygen and cyclosporin A in the buffer solution.

Details

show
hide
Language(s):
 Dates: 2002-03
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1097/00004647-200203000-00012
BibTex Citekey: SerkovaDGHNBBLC2002
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Journal of Cerebral Blood Flow and Metabolism
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: New York : Lippincott Williams & Wilkins
Pages: - Volume / Issue: 22 (3) Sequence Number: - Start / End Page: 342 - 352 Identifier: ISSN: 0271-678X
CoNE: https://pure.mpg.de/cone/journals/resource/954925503202