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  Metabolic changes in quinolinic acid-lesioned rat striatum measured non-invasively by in vivo 1H NMR spectroscopy

Tkac, I., Keene, C., Pfeuffer, J., Low, W., & Gruetter, R. (2001). Metabolic changes in quinolinic acid-lesioned rat striatum measured non-invasively by in vivo 1H NMR spectroscopy. Journal of Neuroscience Research, 66(5), 891-898. doi:10.1002/jnr.10112.

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Tkac, I, Autor
Keene, CD, Autor
Pfeuffer, J1, Autor           
Low, WC, Autor
Gruetter, R, Autor
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1External Organizations, ou_persistent22              

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 Zusammenfassung: Intrastriatal injection of quinolinic acid (QA) provides an animal model of Huntington disease. In vivo (1)H NMR spectroscopy was used to measure the neurochemical profile non-invasively in seven animals 5 days after unilateral injection of 150 nmol of QA. Concentration changes of 16 metabolites were measured from 22 microl volume at 9.4 T. The increase of glutamine ((+25 +/- 14), mean +/- SD, n = 7) and decrease of glutamate (-12 +/- 5), N-acetylaspartate (-17 +/- 6), taurine (-14 +/- 6) and total creatine (-9 +/- 3) were discernible in each individual animal (P < 0.005, paired t-test). Metabolite concentrations in control striata were in excellent agreement with biochemical literature. The change in glutamate plus glutamine was not significant, implying a shift in the glutamate-glutamine interconversion, consistent with a metabolic defect at the level of neuronal-glial metabolic trafficking. The most significant indicator of the lesion, however, were the changes in glutathione ((-19 +/- 9), P < 0.002)), consistent with oxidative stress. From a comparison with biochemical literature we conclude that high-resolution in vivo (1)H NMR spectroscopy accurately reflects the neurochemical changes induced by a relatively modest dose of QA, which permits one to longitudinally follow mitochondrial function, oxidative stress and glial-neuronal metabolic trafficking as well as the effects of treatment in this model of Huntington disease.

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 Datum: 2001-12
 Publikationsstatus: Erschienen
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 Identifikatoren: BibTex Citekey: 2041
DOI: 10.1002/jnr.10112
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Titel: Journal of Neuroscience Research
  Andere : J. Neurosci. Res.
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: Hoboken, NJ : Wiley-Liss, <2005->
Seiten: - Band / Heft: 66 (5) Artikelnummer: - Start- / Endseite: 891 - 898 Identifikator: ISSN: 0360-4012
CoNE: https://pure.mpg.de/cone/journals/resource/954925521676