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Schlagwörter:
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Zusammenfassung:
Drosophila transient receptor potential (TRP) is a prototypical member of a novel family of channel proteins underlying
phosphoinositide-mediated Ca2+ entry. Although the initial stages of this signaling cascade are well known, downstream events leading to the opening of the
TRP channels are still obscure. In the present study we applied patch-clamp whole-cell recordings and measurements of Ca2+ concentration by ion-
selective microelectrodes in eyes of normal and mutant Drosophila to isolate the TRP and TRP-like (TRPL)-dependent currents. We report that anoxia
rapidly and reversibly depolarizes the photoreceptors and induces Ca2+ influx into these cells in the dark. We further show that openings of the light-sensitive
channels, which mediate these effects, can be obtained by mitochondrial uncouplers or by depletion of ATP in photoreceptor cells, whereas the effects of
illumination and all forms of metabolic stress were additive. Effects similar to those found in wild-type flies were also found in mutants with strong defects in
rhodopsin, Gq-protein, or phospholipase C, thus indicating that the metabolic stress operates at a late stage of the phototransduction cascade. Genetic
elimination of both TRP and TRPL channels prevented the effects of anoxia, mitochondrial uncouplers, and depletion of ATP, thus demonstrating that the
TRP and TRPL channels are specific targets of metabolic stress. These results shed new light on the properties of the TRP and TRPL channels by showing
that a constitutive ATP-dependent process is required to keep these channels closed in the dark, a requirement that would make them sensitive to metabolic
stress.
