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  Freeze-substitution of Drososphila heads for subsequent 3H-2-deoxyglucose autoradiography

Rodrigues, V., & Bülthoff, I. (1985). Freeze-substitution of Drososphila heads for subsequent 3H-2-deoxyglucose autoradiography. Journal of Neuroscience Methods, 13(3-4), 183-190. doi:10.1016/0165-0270(85)90066-4.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-F005-D Version Permalink: http://hdl.handle.net/21.11116/0000-0005-E8C2-A
Genre: Journal Article

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 Creators:
Rodrigues, V1, 2, Author              
Bülthoff, I1, 2, Author              
Affiliations:
1Former Department Neurophysiology of Insect Behavior, Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497802              
2Max Planck Institute for Biological Cybernetics, Max Planck Society, Spemannstrasse 38, 72076 Tübingen, DE, ou_1497794              

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 Abstract: High resolution of [3H]2-deoxyglucose labelling was obtained in autoradiographs of Drosophila brains after freeze-substitution in anhydrous acetone at −76°C. This method was applied to preparations which received visual, olfactory and mechanosensory stimulation. The autoradiographs were compared to those obtained after freeze-drying. Freeze-substitution, which has proved to be technically simple, rapid and inexpensive, yields a good quality of tissue preservation and hence is recommended for tissue dehydration prior to autoradiography.

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 Dates: 1985-05
 Publication Status: Published in print
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 Rev. Type: -
 Identifiers: DOI: 10.1016/0165-0270(85)90066-4
BibTex Citekey: 1129
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Title: Journal of Neuroscience Methods
  Other : J. Neurosci. Meth.
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 13 (3-4) Sequence Number: - Start / End Page: 183 - 190 Identifier: ISSN: 0165-0270
CoNE: https://pure.mpg.de/cone/journals/resource/954925480594