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  Direct proteomic quantification of the secretome of activated immune cells

Meissner, F., Scheltema, R. A., Mollenkopf, H.-J., & Mann, M. (2013). Direct proteomic quantification of the secretome of activated immune cells. Science, 340(6131), 475-478. doi:10.1126/science.1232578.

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 Creators:
Meissner, Felix1, Author              
Scheltema, Richard A.1, Author              
Mollenkopf, Hans-Joachim2, Author              
Mann, Matthias1, Author              
Affiliations:
1Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565159              
2Core Facilities / Microarray, Max Planck Institute for Infection Biology, Max Planck Society, ou_1664141              

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 Abstract: Protein secretion allows communication of distant cells in an organism and controls a broad range of physiological functions. We describe a quantitative, high-resolution mass spectrometric workflow to detect and quantify proteins that are released from immune cells upon receptor ligation. We quantified the time-resolved release of 775 proteins, including 52 annotated cytokines from only 150,000 primary Toll-like receptor 4–activated macrophages per condition. Achieving low picogram sensitivity, we detected secreted proteins whose abundance increased by a factor of more than 10,000 upon stimulation. Secretome to transcriptome comparisons revealed the transcriptionally decoupled release of lysosomal proteins. From genetic models, we defined secretory profiles that depended on distinct intracellular signaling adaptors and showed that secretion of many proinflammatory proteins is safeguarded by redundant mechanisms, whereas signaling adaptor synergy promoted the release of anti-inflammatory proteins.

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Language(s): eng - English
 Dates: 2013-04-26
 Publication Status: Published in print
 Pages: 4
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1126/science.1232578
 Degree: -

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Title: Science
Source Genre: Journal
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Publ. Info: Washington, D.C. : American Association for the Advancement of Science
Pages: - Volume / Issue: 340 (6131) Sequence Number: - Start / End Page: 475 - 478 Identifier: ISSN: 0036-8075
CoNE: https://pure.mpg.de/cone/journals/resource/991042748276600_1