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  Membrane and actin reorganization in electropulse-induced cell fusion

Gerisch, G., Ecke, M., Neujahr, R., Prassler, J., Stengl, A., Hoffmann, M., et al. (2013). Membrane and actin reorganization in electropulse-induced cell fusion. JOURNAL OF CELL SCIENCE, 126(9), 2069-2078. doi:10.1242/jcs.124073.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-F663-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-184F-6
Genre: Journal Article

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 Creators:
Gerisch, Günther1, Author              
Ecke, Mary1, Author              
Neujahr, Ralph1, Author              
Prassler, Jana1, Author              
Stengl, Andreas1, Author              
Hoffmann, Max2, Author
Schwarz, Ulrich S.2, Author
Neumann, Eberhard2, Author
Affiliations:
1Gerisch, Günther / Cell Dynamics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565149              
2external, ou_persistent22              

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Free keywords: GREEN FLUORESCENT PROTEIN; ELECTRIC-FIELD PULSE; VESICLE FUSION; CURVATURE; DICTYOSTELIUM; PHASE; CYTOSKELETON; TRANSITION; DIFFUSION; BILAYERSActin polymerization; Cell repair; Diffusion; Electric lipid distortion; Fusion pores; Membrane fusion;
 Abstract: When cells of Dictyostelium discoideum are exposed to electric pulses they are induced to fuse, yielding motile polykaryotic cells. By combining electron microscopy and direct recording of fluorescent cells, we have studied the emergence of fusion pores in the membranes and the localization of actin to the cell cortex. In response to electric pulsing, the plasma membranes of two contiguous cells are turned into tangles of highly bent and interdigitated membranes. Live-imaging of cells double-labeled for membranes and filamentous actin revealed that actin is induced to polymerize in the fusion zone to temporarily bridge the gaps in the vesiculating membrane. The diffusion of green fluorescent protein (GFP) from one fusion partner to the other was scored using spinning disc confocal microscopy. Fusion pores that allowed intercellular exchange of GFP were formed after a delay, which lasted up to 24 seconds after exposure of the cells to the electric field. These data indicate that the membranes persist in a fusogenic state before pores of about 3 nm diameter are formed.

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Language(s): eng - English
 Dates: 2013-05-01
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: ISI: 000319561700018
DOI: 10.1242/jcs.124073
 Degree: -

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Title: JOURNAL OF CELL SCIENCE
Source Genre: Journal
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Publ. Info: BIDDER BUILDING CAMBRIDGE COMMERCIAL PARK COWLEY RD, CAMBRIDGE CB4 4DL, CAMBS, ENGLAND : COMPANY OF BIOLOGISTS LTD
Pages: - Volume / Issue: 126 (9) Sequence Number: - Start / End Page: 2069 - 2078 Identifier: ISSN: 0021-9533