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  The chromodomain helicase Chd4 is required for Polycomb-mediated inhibition of astroglial differentiation

Sparmann, A., Xie, Y., Verhoeven, E., Vermeulen, M., Lancini, C., Gargiulo, G., et al. (2013). The chromodomain helicase Chd4 is required for Polycomb-mediated inhibition of astroglial differentiation. EMBO JOURNAL, 32(11), 1598-1612. doi:10.1038/emboj.2013.93.

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 Creators:
Sparmann, Anke1, Author
Xie, Yunli1, Author
Verhoeven, Els1, Author
Vermeulen, Michiel2, Author              
Lancini, Cesare1, Author
Gargiulo, Gaetano1, Author
Hulsman, Danielle1, Author
Mann, Matthias2, Author              
Knoblich, Juergen A.1, Author
van Lohuizen, Maarten1, Author
Affiliations:
1external, ou_persistent22              
2Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565159              

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Free keywords: CHROMATIN REMODELER MI-2-BETA; HEMATOPOIETIC STEM-CELLS; GROUP GENE EZH2; DNA METHYLATION; HISTONE DEACETYLASE; SELF-RENEWAL; ASTROCYTE DIFFERENTIATION; DEVELOPMENTAL REGULATORS; MOUSE DEVELOPMENT; NURD COMPLEXastrogenesis; Chd4; differentiation; neural stem cells; polycomb;
 Abstract: Polycomb group (PcG) proteins form transcriptional repressor complexes with well-established functions during cell-fate determination. Yet, the mechanisms underlying their regulation remain poorly understood. Here, we extend the role of Polycomb complexes in the temporal control of neural progenitor cell (NPC) commitment by demonstrating that the PcG protein Ezh2 is necessary to prevent the premature onset of gliogenesis. In addition, we identify the chromodomain helicase DNA-binding protein 4 (Chd4) as a critical interaction partner of Ezh2 required specifically for PcG-mediated suppression of the key astrogenic marker gene GFAP. Accordingly, in vivo depletion of Chd4 in the developing neocortex promotes astrogenesis. Collectively, these results demonstrate that PcG proteins operate in a highly dynamic, developmental stage-dependent fashion during neural differentiation and suggest that target gene-specific mechanisms regulate Polycomb function during sequential cell-fate decisions.

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Language(s): eng - English
 Dates: 2013-05-29
 Publication Status: Published in print
 Pages: 15
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000319562800011
DOI: 10.1038/emboj.2013.93
 Degree: -

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Title: EMBO JOURNAL
Source Genre: Journal
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Publ. Info: 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA : NATURE PUBLISHING GROUP
Pages: - Volume / Issue: 32 (11) Sequence Number: - Start / End Page: 1598 - 1612 Identifier: ISSN: 0261-4189