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  Fast neurotransmitter release regulated by the endocytic scaffold intersectin.

Sakaba, T., Kononenko, N. L., Bacetic, J., Pechstein, A., Schmoranzer, J., Yao, L., et al. (2013). Fast neurotransmitter release regulated by the endocytic scaffold intersectin. Proccedings of the National Academy of Sciences of the United States of America, 110(20), 8266-8271. doi:10.1073/pnas.1219234110.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-F84E-5 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-C800-6
Genre: Journal Article

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1798434.pdf (Publisher version), 2MB
 
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 Creators:
Sakaba, T.1, Author              
Kononenko, N. L., Author
Bacetic, J., Author
Pechstein, A., Author
Schmoranzer, J., Author
Yao, L., Author
Barth, H., Author
Shupliakov, O., Author
Kobler, O., Author
Aktories, K., Author
Haucke, V., Author
Affiliations:
1Research Group of Biophysics of Synaptic Transmission, MPI for biophysical chemistry, Max Planck Society, ou_578583              

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Free keywords: endocytosis; synaptic transmission; synaptic vesicle recruitment
 Abstract: Sustained fast neurotransmission requires the rapid replenishment of release-ready synaptic vesicles (SVs) at presynaptic active zones. Although the machineries for exocytic fusion and for subsequent endocytic membrane retrieval have been well characterized, little is known about the mechanisms underlying the rapid recruitment of SVs to release sites. Here we show that the Down syndrome-associated endocytic scaffold protein intersectin 1 is a crucial factor for the recruitment of release-ready SVs. Genetic deletion of intersectin 1 expression or acute interference with intersectin function inhibited the replenishment of release-ready vesicles, resulting in short-term depression, without significantly affecting the rate of endocytic membrane retrieval. Acute perturbation experiments suggest that intersectin-mediated vesicle replenishment involves the association of intersectin with the fissioning enzyme dynamin and with the actin regulatory GTPase CDC42. Our data indicate a role for the endocytic scaffold intersectin in fast neurotransmitter release, which may be of prime importance for information processing in the brain.

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Language(s): eng - English
 Dates: 2013-04-302013-05-14
 Publication Status: Published in print
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1073/pnas.1219234110
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Title: Proccedings of the National Academy of Sciences of the United States of America
Source Genre: Journal
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Pages: - Volume / Issue: 110 (20) Sequence Number: - Start / End Page: 8266 - 8271 Identifier: -