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  Real-time in vivo analysis of T cell activation in the central nervous system using a genetically encoded calcium indicator

Mues, M., Bartholomäus, I., Thestrup, T., Griesbeck, O., Wekerle, H., Kawakami, N., et al. (2013). Real-time in vivo analysis of T cell activation in the central nervous system using a genetically encoded calcium indicator. NATURE MEDICINE, 19(6), 778-783. doi:10.1038/nm.3180.

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 Creators:
Mues, Marsilius1, Author              
Bartholomäus, Ingo1, Author              
Thestrup, Thomas2, Author              
Griesbeck, Oliver2, Author              
Wekerle, Hartmut1, Author              
Kawakami, Naoto1, Author              
Krishnamoorthy, Gurumoorthy1, Author              
Affiliations:
1Emeritus Group: Neuroimmunology / Wekerle, MPI of Neurobiology, Max Planck Society, ou_1113547              
2Research Group: Cellular Dynamics / Griesbeck, MPI of Neurobiology, Max Planck Society, ou_1113560              

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Free keywords: GREEN-FLUORESCENT PROTEIN; AUTOIMMUNE CNS LESIONS; DENDRITIC CELLS; TRANSGENIC MICE; LYMPH-NODES; LYMPHOCYTES; ANTIGEN; SIGNALS; DISEASE
 Abstract: To study T cell activation in vivo in real time, we introduced a newly developed fluorescence resonance energy transfer-based, genetically encoded calcium indicator into autoantigen-specific and non-autoantigen-specific CD4(+) T cells. Using two-photon microscopy, we explored the responses of retrovirally transduced calcium indicator-expressing T cells to antigen in the lymph nodes and the central nervous system. In lymph nodes, the administration of exogenous antigen caused an almost immediate arrest of T cells around antigen-presenting cells and an instant rise of cytosolic calcium. In contrast, encephalitogenic T cells entering the leptomeningeal space, one main portal into the central nervous system parenchyma during experimental autoimmune encephalomyelitis, showed elevated intracellular calcium concentrations while still meandering through the space. This approach enabled us to follow the migration and activation patterns of T cells in vivo during the course of the disease.

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Language(s): eng - English
 Dates: 2013-06
 Publication Status: Published in print
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000319981600029
DOI: 10.1038/nm.3180
 Degree: -

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Title: NATURE MEDICINE
Source Genre: Journal
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Publ. Info: 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA : NATURE PUBLISHING GROUP
Pages: - Volume / Issue: 19 (6) Sequence Number: - Start / End Page: 778 - 783 Identifier: ISSN: 1078-8956