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  Real-time in vivo analysis of T cell activation in the central nervous system using a genetically encoded calcium indicator

Mues, M., Bartholomäus, I., Thestrup, T., Griesbeck, O., Wekerle, H., Kawakami, N., et al. (2013). Real-time in vivo analysis of T cell activation in the central nervous system using a genetically encoded calcium indicator. NATURE MEDICINE, 19(6), 778-783. doi:10.1038/nm.3180.

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Mues, Marsilius1, Autor           
Bartholomäus, Ingo1, Autor           
Thestrup, Thomas2, Autor           
Griesbeck, Oliver2, Autor           
Wekerle, Hartmut1, Autor           
Kawakami, Naoto1, Autor           
Krishnamoorthy, Gurumoorthy1, Autor           
Affiliations:
1Emeritus Group: Neuroimmunology / Wekerle, MPI of Neurobiology, Max Planck Society, ou_1113547              
2Research Group: Cellular Dynamics / Griesbeck, MPI of Neurobiology, Max Planck Society, ou_1113560              

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Schlagwörter: GREEN-FLUORESCENT PROTEIN; AUTOIMMUNE CNS LESIONS; DENDRITIC CELLS; TRANSGENIC MICE; LYMPH-NODES; LYMPHOCYTES; ANTIGEN; SIGNALS; DISEASE
 Zusammenfassung: To study T cell activation in vivo in real time, we introduced a newly developed fluorescence resonance energy transfer-based, genetically encoded calcium indicator into autoantigen-specific and non-autoantigen-specific CD4(+) T cells. Using two-photon microscopy, we explored the responses of retrovirally transduced calcium indicator-expressing T cells to antigen in the lymph nodes and the central nervous system. In lymph nodes, the administration of exogenous antigen caused an almost immediate arrest of T cells around antigen-presenting cells and an instant rise of cytosolic calcium. In contrast, encephalitogenic T cells entering the leptomeningeal space, one main portal into the central nervous system parenchyma during experimental autoimmune encephalomyelitis, showed elevated intracellular calcium concentrations while still meandering through the space. This approach enabled us to follow the migration and activation patterns of T cells in vivo during the course of the disease.

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Sprache(n): eng - English
 Datum: 2013-06
 Publikationsstatus: Erschienen
 Seiten: 6
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: ISI: 000319981600029
DOI: 10.1038/nm.3180
 Art des Abschluß: -

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Titel: NATURE MEDICINE
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA : NATURE PUBLISHING GROUP
Seiten: - Band / Heft: 19 (6) Artikelnummer: - Start- / Endseite: 778 - 783 Identifikator: ISSN: 1078-8956